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| 利用cDNA-AFLP分析大白菜、萝卜及其杂交种差异表达基因 | |
| 引用本文： | 张庶,李利斌,王凤德,刘立锋,李化银,高建伟,高玲.利用cDNA-AFLP分析大白菜、萝卜及其杂交种差异表达基因[J].山东农业科学,2011(7):1-4,8. |
| 作者姓名： | 张庶 李利斌 王凤德 刘立锋 李化银 高建伟 高玲 |
| 作者单位： | 1. 青岛农业大学生命科学学院,山东青岛266109;山东省农业科学院蔬菜研究所;山东省设施蔬菜生物学重点实验室;国家蔬菜改良中心山东分中心,山东济南250100 2. 山东省农业科学院蔬菜研究所;山东省设施蔬菜生物学重点实验室;国家蔬菜改良中心山东分中心,山东济南250100 3. 青岛农业大学生命科学学院,山东青岛,266109 |
| 基金项目： | 国家“973”前期研究专项(编号:2009CB126002); 山东省现代农业产业技术体系——蔬菜体系项目; 山东省优秀中青年科学家科研奖励基金(编号:BS2010SW027)资助 |
| 摘 要： | 为更好地理解十字花科蔬菜杂种优势的分子机理,对大白菜(Brassica rapa L.ssp.pekinensis)、萝卜(Raphanus sativus L.)及其杂交种的差异表达基因进行了初步研究.利用cDNA-AFLP技术对杂种F1及其双亲进行基因差异表达分析,共得到44个在杂种F1特异表达的差异片段,分属40... |
| 关 键 词： | 基因差异表达 大白菜 萝卜 杂种F1 cDNA-AFLP |
Analysis of Differentially Expressed Genes in Brassica rapa L.ssp. pekinensis,Raphanus sativus L. and Their Hybrid Using cDNA-AFLP |
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| ZHANG Shu,LI Li-bin,WANG Feng-de,LIU Li-feng,LI Hua-yin,GAO Jian-wei,GAO Ling.Analysis of Differentially Expressed Genes in Brassica rapa L.ssp. pekinensis,Raphanus sativus L. and Their Hybrid Using cDNA-AFLP[J].Shandong Agricultural Sciences,2011(7):1-4,8. | |
| Authors: | ZHANG Shu LI Li-bin WANG Feng-de LIU Li-feng LI Hua-yin GAO Jian-wei GAO Ling |
| Institution: | ZHANG Shu1,2,LI Li-bin2,WANG Feng-de2,LIU Li-feng2,LI Hua-yin2,GAO Jian-wei1,2 GAO Ling1(1.College of Life Science,Qingdao Agricultural University,Qingdao 266109,China,2.Vegetable Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China) |
| Abstract: | For well understand the molecular mechanism of heterosis in Cruciferae vegetables,the differentially expressed genes in Chinese cabbage(Brassica rapa L.ssp.pekinensis) Fushanbaotou,radish(Raphanus sativus L.) Tiaotouqing and their F1 hybrid were displayed by cDNA-AFLP.And 44 differential bands in F1 hybrid were obtained,which belonged to 40 unigenes.Among them,43 bands could be found homologous sequences in NCBI/GenBank and only 32 bands,function had been known.According to sequence analysis,the function-kn... |
| Keywords: | Differential gene expression Brassica rapa L ssp pekinensis Raphanus sativus L Hybrid F1 cDNA-AFLP |
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