| 一株高效秸秆纤维素降解真菌的分离、鉴定及系统发育分析 |
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| 引用本文： | 李方正,唐亮,赵建文,王文娟,赵宏坤.一株高效秸秆纤维素降解真菌的分离、鉴定及系统发育分析[J].山东农业科学,2011(7):5-8. |
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| 作者姓名： | 李方正 唐亮 赵建文 王文娟 赵宏坤 |
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| 作者单位： | 1. 山东农业大学动物科技学院,山东泰安271018;
2. 山东农业大学动物科技学院,山东泰安271018;青岛农业大学食品科学与工程学院;山东省肉品质量安全控制工程技术研究中心,山东青岛266109 |
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| 基金项目： | 山东省肉品质量安全控制工程技术研究中心组建项目资助 |
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| 摘 要： | 通过刚果红染色法初筛，结合产酶活性测定复筛，从奶牛、山羊瘤胃内容物中分离到一株纤维素高效降解菌NL-3，克隆了该菌的18SrDNA序列，并以其同源性为基础构建了相关属种的系统发育树。结果表明，18SrDNA序列全长504bp，Blast显示该菌株与青霉菌属同源；NL-3菌株在进化关系上也与青霉菌属（Penicillium）聚成一族。特别是与Penicillium decumbens strain JU - A10的同源性最高，序列相似性达99％。结合菌株形态学及18SrDNA基因序列分析结果对菌株进行鉴定，初步鉴定为青霉属的斜卧青霉（Penicillium decumbens）。
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| 关 键 词： | 纤维素降解真菌 鉴定 系统发育分析 |
Isolation,Identification and Phylogenetic Analysis of an Efficient Straw Cellulose-Degrading Fungus |
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| LI Fang-zheng,TANG Liang,ZHAO Jian-wen,WANG Wen-juan,ZHAO Hong-kun.Isolation,Identification and Phylogenetic Analysis of an Efficient Straw Cellulose-Degrading Fungus[J].Shandong Agricultural Sciences,2011(7):5-8. |
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| Authors: | LI Fang-zheng TANG Liang ZHAO Jian-wen WANG Wen-juan ZHAO Hong-kun |
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| Institution: | LI Fang-zheng1,TANG Liang1,ZHAO Jian-wen1,WANG Wen-juan1,ZHAO Hong-kun1,2(1.College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,China,2.College of Food Science and Engineering,Qingdao Agricultural University/ Shandong Engineering Center for Meat Food Quality Safety Control,Qingdao 266109,China) |
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| Abstract: | A fungal strain named as NL-3 that could degrade cellulose was isolated from the rumen of cows and sheep.The 18S rDNA gene sequence for strain NL-3 was amplified and sequenced.The results showed that the complete 18S rDNA gene sequence was 504 bp.A phylogenetic tree was constructed by comparing the 18S rDNA sequence with other relative fungus species in the GenBank database.The strain NL-3 and Penicillium decumbens strain JU-A10 constituted a branch with the similarity value as 99%.According to the morpholo... |
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| Keywords: | Cellulose-degrading fungus Identification Phylogenetic analysis |
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