青州蜜桃、冬桃成熟胚子叶再生不定植株的研究

以青州蜜桃、冬桃成熟胚子叶为外植体 ,研究了利用子叶再生不定梢的适宜培养基。两个品种的子叶在 1 / 2MS附加BA 1 0mg/L和NAA 0 .2mg/L的培养基上获得了最高不定梢再生率。青州蜜桃和冬桃子叶的不定梢最高再生率分别是 6 6 .7%和 5 6 .2 %。不定梢在WPM +BA 2mg/L +IBA 0 .0 1mg/L +GA 0 .5mg/L培养基上增殖培养后 ,转移到 1 / 2MS +IBA 0 .5mg/L +蔗糖 2 0g/L诱导生根 ,两个品种的生根率都在 90 %以上

Plant regeneration from cotyledons of mature seed of two peach cultivars

Cotyledons of mature seed from two cultivars “Qingzhou honey peach”and “Winter peach” were used as explant.The suitable media inducing shoot regeneration from cotyledons was studied.Shoot regeneration rates were highest when cotyledons were cultured on 1/2MS with BA 10mg/L and NAA 0.2mg/L.Regeneration rates for Qingzhou honey peach and Winter peach were 66.7% and 56.2% respectively.Shoots were proliferated on medium WPM with BA 2mg/L,IBA 0.01mg/L and GA 0.5mg/L.To induce root,shoots were transferred from proliferation medium to root induction medium.Rooting rates of two cultivars were above 90%on 1/2MS medium with 20g/L sucrose and IBA 0.5mg/L.