| 富铬酿酒酵母的单倍体原生质体复合诱变选育 |
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| 引用本文： | 张瞳,贤一博,张璐,许峰,贾乐.富铬酿酒酵母的单倍体原生质体复合诱变选育[J].山东农业科学,2006(2):35-38. |
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| 作者姓名： | 张瞳 贤一博 张璐 许峰 贾乐 |
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| 作者单位： | 山东农业大学生命科学学院,山东,泰安,271018 |
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| 摘 要： | 对酿酒酵母(Saccharomyces cerevisiae)单倍体菌株SY8的原生质体进行紫外线(UV)和微波(MW)复合诱变,获得1株高抗性突变菌株SY8-4-29,其Cr3+抗性水平为20 mmol/L,是出发菌株(5 mmol/L)的4倍,其铬含量为6.211 mg/kg,约是出发菌株(1.265 mg/kg)的5倍,拮抗紫外线6、0Co辐射和清除羟基自由基的能力显著增强。50世代培养结果表明,其遗传稳定性在95%以上。
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| 关 键 词： | 酿酒酵母 原生质体 单倍体 复合诱变 |
| 文章编号： | 1001-4942（2006）02-0035-04 |
| 收稿时间： | 2005-12-12 |
| 修稿时间： | 2005年12月12 |
Multi-mutated breeding of haploid protoplast of Saccharomyces cerevisiae enrichment chromium |
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| ZHANG Tong,XIAN Yi-bo,ZHANG Lu,XU Feng,JIA Le.Multi-mutated breeding of haploid protoplast of Saccharomyces cerevisiae enrichment chromium[J].Shandong Agricultural Sciences,2006(2):35-38. |
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| Authors: | ZHANG Tong XIAN Yi-bo ZHANG Lu XU Feng JIA Le |
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| Institution: | College of Life Sciences, Shandong Agricultural University, Taian 271018. China |
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| Abstract: | The haploid strain SY8 of Saccharomyces cerevisiae was multi-mutated with UV and Microwave.Mutant strain SY8-4-29 was obtained,whose resistance level to chromium reached 20 mmol/L;chromium content was about fivefold higher than that in the initial strain;biological function was notably improved,and genetic stability after 50 generations was over 95%. |
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| Keywords: | Saccharomyces cerevisiae Haploid Protoplast Multi-mutated |
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