棉花T—DNA标签雄性不育突变体的遗传分析

利用农杆菌介导T—DNA插入得到棉花雄性不育突变体，与野生型陆地棉（Gossypium hirsutum L）Coker 312杂交得到F1代。F1代植株出现了不育与可育两种性状的分离，分离比是1：1。对F1代进行形态学观察、卡那霉素和除草剂抗性鉴定、PCR扩增检测以及遗传学分析，证实雄性不育突变性状的表现与T—DNA插入共分离，从而认为突变是由T—DNA插入引起的显性杂合突变，这为利用T—DNA标签法进行雄性不育有关基因的克隆奠定了基础。

Genetic Analysis of Cotton Male Sterile Mutant by T-DNA Tagging

A male sterile mutant by T-DNA insertion was crossed with a wild type upland cotton(Gossypium hirsutum L.) cultivar Coker312.The segregation ratio of male sterile to male fertile was 1∶1 in generation F1.The results of morphological observation,kanamycin and herbicide resistance assay,PCR identification and corresponding genetic analysis showed that the male sterile was co-segregated with the T-DNA insertion.This male sterile mutant was considered as dominant heterozygous mutation caused by the T-DNA insertion,which would lay the basis for cloning the male sterile related genes of upland cotton by T-DNA tagging.