| 农杆菌介导的薹菜遗传转化体系研究 |
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| 引用本文： | 许会会,赵美爱,钤泰琳,刘维信.农杆菌介导的薹菜遗传转化体系研究[J].山东农业科学,2011(5):8-11. |
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| 作者姓名： | 许会会 赵美爱 钤泰琳 刘维信 |
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| 作者单位： | 1. 青岛农业大学园林园艺学院,山东,青岛,266109
2. 青岛农业大学生命科学学院,山东,青岛,266109 |
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| 基金项目： | 国家科技支撑计划项目资助(2008BADB1B01); 山东省自然科学基金资助项目(Y2007D26) |
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| 摘 要： | 以薹菜子叶-子叶柄为外植体,研究了根癌农杆菌介导的遗传转化技术。用含GUS和NPTⅡ基因的农杆菌侵染子叶-子叶柄,获得了具有卡那霉素抗性的再生植株。再生植株经GUS组织染色,证实外源基因转化到植物基因组中。卡那霉素敏感性测定结果表明,当培养基中含卡那霉素（Km）15 mg/L时,非转化再生植株全部白化。转基因抗性芽筛选的适宜的卡那霉素浓度为10 mg/L。
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| 关 键 词： | 薹菜 子叶-子叶柄 遗传转化 |
An Agrobacterium-mediated Transformation System for Brassica campetris L.ssp.chinensis Makino var.tai-tsai Hort. |
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| XU Hui-hui,ZHAO Mei-ai,QIAN Tai-lin,LIU Wei-xin.An Agrobacterium-mediated Transformation System for Brassica campetris L.ssp.chinensis Makino var.tai-tsai Hort.[J].Shandong Agricultural Sciences,2011(5):8-11. |
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| Authors: | XU Hui-hui ZHAO Mei-ai QIAN Tai-lin LIU Wei-xin |
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| Institution: | XU Hui-hui1,ZHAO Mei-ai2,QIAN Tai-lin2,LIU Wei-xin1(1.College of Landscape & Horticulture,Qingdao Agricultural University,Qingdao 266109,China,2.College of Life Science,China) |
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| Abstract: | A genetic transformation system mediated by Agrobacterium tumefaciens was developed for Brassica campetris L.ssp.chinensis Makino var.tai-tsai Hort.using petioles with cotyledon as explant.The Agrobacterium tumefaciens carrying GUS and NPTⅡ genes was used for transformation.The plantlets resistant to kanamycin were obtained.GUS histochemical assay proved that GUS gene had been stably integrated into the chromosomal genome of Brassica campetris L.ssp.chinensis Makino var.tai-tsai Hort.The test showed that all the nontransformed shoots turned white on the medium with 15 mg/L kanamycin.Therefore,10 mg/L kanamycin was suitable for the selection of transformed shoots. |
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| Keywords: | Brassica campetris L ssp chinensis Makino var tai-tsai Hort Petioles with cotyledon Genetic transformation |
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