西畴乌骨鸡mtDNA D-loop区遗传多样性分析*

 为了从母系遗传角度深入阐明西畴乌骨鸡的群体遗传背景，采用PCR直接测序法测定了36只西畴乌骨鸡的线粒体DNA D-loop区第I高变区520 bp序列。结果表明：在所分析的西畴乌骨鸡D-loop区序列中，T，C，A和G平均含量分别为30.3%，29.7%，27.4%和12.6%；共检测到核苷酸变异位点26个，核苷酸多样度为0.01452±0.00098；D-loop区序列存在12种单倍型，单倍型多样度为0.884±0.027，表明西畴乌骨鸡遗传多样性较丰富。聚类分析显示西畴乌骨鸡聚为A，B两大支，其中，A世系分为3小支，B世系分为2小支，说明西畴乌骨鸡在母系血统上存在较大遗传分化。

Genetic Diversity of Mitochondrial DNA D-loop Region of Xichou Black-bone Chicken

In the present study, 36 Xichou black bone chickens were selected from Xichou County, Yunnan province The 520 bp HVS I region of mitochondrial DNA D loop was determined by direct sequencing of PCR products The results revealed that the average percentage of T, C, A and G were 30.3%，29.7%，27.4% and 12.6%, respectivelyTotally, 26 mutation sites and 12 haplotypes were detectedThe nucleotide diversity was 0.01452±0.00098, and the haplotype diversity was 0.884±0.027 Phylogenetic analysis by NJ method showed that all the sequences were clustered in 2 major clades: A clade which was further divided into 3 subclades and B clade which was further divided into 2 subclades The results in this study indicated that there was remarkably genetic differentiation for the maternal origin of Xichou black bone chicken