| 昌红苹果离体叶片不定芽的诱导及植株再生 |
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| 引用本文： | 吴雅琴,赵艳华,李春敏.昌红苹果离体叶片不定芽的诱导及植株再生[J].云南农业大学学报,2006,21(1):32-35. |
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| 作者姓名： | 吴雅琴 赵艳华 李春敏 |
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| 作者单位： | 河北省农林科学院吕黎果树研究所,河北吕黎066600 |
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| 摘 要： | 以昌红苹果的组培苗为试材,研究建立叶片的离体再生体系。用30~40 d继代的新梢顶部1~3节新展开的叶片作为外植体,分别接种在分化的培养基上,黑暗处理15 d, 昌红苹果叶片不定芽的最高再生率为100%。在6号培养基上,昌红苹果叶片的再生能力最高; 诱导叶片不定芽适宜的培养基为MS+TDZ 1.0 mg /L+NAA 0.5 mg/L+蔗糖 30 g/L+ 琼脂 5.5 g/L。
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| 关 键 词： | 苹果 离体叶片 再生体系 组织培养 |
| 文章编号： | 1004-390X（2006）01-0032-04 |
| 收稿时间： | 2005-05-27 |
| 修稿时间： | 2005-05-27 |
Efficient Adventitous Bud Induction and Plant Regeneration from Leaves in Vitro of Apple (cv. Changhong) |
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| WU Ya-qin,ZHAO Yan-hua,LI Chun-min.Efficient Adventitous Bud Induction and Plant Regeneration from Leaves in Vitro of Apple (cv. Changhong)[J].Journal of Yunnan Agricultural University,2006,21(1):32-35. |
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| Authors: | WU Ya-qin ZHAO Yan-hua LI Chun-min |
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| Institution: | Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Science, Changli 066600, China |
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| Abstract: | Factors that influence the shoot regeneration of Apple (cv. Changhong) in vitro leaves were studied. With the first 1~3 new leaves of young shoots that 30~40 d after subculture as explant, about 100% bud regeneration rate was obtained. When tha explant were cultured on the differentiation medium by 15 d dark treatment. The results showed that with No.6 medium, the highest shoot regeneration ability was observed. The optimal medium for regeneration is MS+TDZ 1.0 mg /L+NAA 0.5 mg/L+ Sucrose 30 g/L+ Agar 5.5 g/L. |
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| Keywords: | apple in vitro leaf regeneration tissue culture |
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