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山羊mtDNA多态性及其遗传结构的研究*
引用本文:季 香,马月辉,叶绍辉,郭 军.山羊mtDNA多态性及其遗传结构的研究*[J].云南农业大学学报,2008,23(2):220-224.
作者姓名:季 香  马月辉  叶绍辉  郭 军
作者单位:中国农业科学院畜牧研究所,中国农业科学院畜牧研究所,云南农业大学动物科学技术学院,中国农业科学院畜牧研究所 北京100094,云南农业大学动物科学技术学院,云南昆明650201,北京100094,云南昆明650201,北京100094
基金项目:国家自然科学基金项目(30371026),国家自然科技资源平台项目(2005DKA21101)
摘    要: 对中国6个山羊品种(辽宁绒山羊,内蒙古绒山羊,西藏山羊,中卫山羊,济宁青山羊和龙陵山羊)的52个样本进行mtDNA的D-Loop区的全序列测定,用来研究中国山羊品种的线粒体DNA遗传多态性及系统发育。用NJ法构建单倍型系统发育树,结果发现6个山羊品种的线粒体DNA控制区聚类后得到了4个很明显的分支,即单倍型A,B,C,D.品种间遗传结构分析发现内蒙古绒山羊、辽宁绒山羊两个绒山羊品种与西藏山羊、中卫山羊3个可产绒的山羊品种聚在一起,接着与产羔皮的济宁青山羊聚在一起,最后与产肉性能强的龙陵山羊聚在一起。

关 键 词:山羊  mtDNA  遗传多样性  系统发育
文章编号:1004-390X(2008)02-0220-05
收稿时间:2007-05-21
修稿时间:2007-09-24

Research on the Genetic Diversity and Structure of mtDNA of 6 Indigenous Goat Breeds in China
JI Xiang,MA Yue-hui,YE Shao-hui,GUO Jun.Research on the Genetic Diversity and Structure of mtDNA of 6 Indigenous Goat Breeds in China[J].Journal of Yunnan Agricultural University,2008,23(2):220-224.
Authors:JI Xiang  MA Yue-hui  YE Shao-hui  GUO Jun
Institution:(1.Institution of Animal Science, China Academy of Agricultural Science, Beijing 100094, China;2.Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China)
Abstract:In this paper,52 individuals were chosen from 6 indigenous goat breeds raised in China.Fifty-two individuals' complete sequences of D-Loop,the control region of mitochondrial DNA(mtDNA),were sequenced in order to study mtDNA genetic diversity and phylogenetic evolution of these 6 breeds.The phylogenetic tree of D-Loop was constructed with Neighbour-Joining(NJ) method and the genetic structure among the 6 breeds was studied by cluster analysis based on the genetic distance.The results showed that all of the domestic haplotypes were divided into 4 high obvious divergent clusters(A,B,C and D) and the cluster analysis showed a closer relationship among LNC,NMC,TiG and ZW goats and a farther relationship between LL goat and other goat.
Keywords:goat  mitochondrial DNA  genetic diversity  phylogeny evolution
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