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烟草黑胫病菌的拮抗根际细菌的筛选
引用本文:方敦煌,顾金刚,李江涛,李天飞,刘杏忠.烟草黑胫病菌的拮抗根际细菌的筛选[J].云南农业大学学报,2001,16(2):93-95.
作者姓名:方敦煌  顾金刚  李江涛  李天飞  刘杏忠
作者单位:[1]云南省烟草科学研究院农业所,云南玉溪653100 [2]中国农业科学院生物防治研究所,北京100081
基金项目:云南省烟草公司项目(98A04)
摘    要: 从重病田健株采集根际土样13份,分离根际细菌753株,室内平皿对峙筛选到拮抗烟草黑胫病菌的菌株98株,占分离菌株的12.7%。温室测定发现有20个菌株防治效果达40.0%以上,超过药剂对照的有4个菌株,防治效果分别为75.7%,78.6%,78.6%,79.3%。其中,大部分菌株对烟草有不同程度的促生作用。

关 键 词:烟草黑胫病  拮抗根际细菌  防治效果
文章编号:1004-390X(2001)02-0093-03
收稿时间:2000-8-21

Screen for Antagonistic Rhizobacteria of Phytophthora nico ti anae on Tobacco
FANG Dun huang ,GU Jin gang ,LI Jiang tao ,LI Tian fei ,LIU Xing zhong.Screen for Antagonistic Rhizobacteria of Phytophthora nico ti anae on Tobacco[J].Journal of Yunnan Agricultural University,2001,16(2):93-95.
Authors:FANG Dun huang  GU Jin gang    LI Jiang tao  LI Tian fei  LIU Xing zhong
Institution:FANG Dun huang 1,GU Jin gang 1,2,LI Jiang tao 3,LI Tian fei 1,LIU Xing zhong 2
Abstract:Thirteen tobacco rhizospere soil samples were collected from healthy plants in heavy disease plots. After isolation and purification, 753 rhizobacteria (RB) were obtained. By paring culture on PDA, 98 antagonistic RB (ARB) to Phytophthrora nictianae (98Pn022) were screened. These antagonistic RB strains were used as a biocontrol agent against black shank in the greenhouse experiment. The results showed 20 strains from 98 strains had a good control effect (40.3%~79.3%).Among of them, 4 strains' relative control efficiency were all above 75%, better than that of compared chemical fungicide. Some ARB can promote tobacco seedling growth in the greenhouse.
Keywords:Tobacco black shank  Antagonistic rhizobacteria (ARB)  Control effect
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