| 木霉拮抗烟草赤星病菌菌株的筛选及其生防机制 |
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| 引用本文： | 王革,周晓罡,方敦煌,李天飞.木霉拮抗烟草赤星病菌菌株的筛选及其生防机制[J].云南农业大学学报,2000,15(3):216-218. |
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| 作者姓名： | 王革 周晓罡 方敦煌 李天飞 |
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| 作者单位： | [1]玉溪红塔烟草集团技术中心,玉溪 [2]云南省烟草研究所,玉溪 |
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| 基金项目： | 云南省自然科学基金资助项目(97C013R) |
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| 摘 要： | 利用诱捕法从土壤中分离得到18株木霉(Trichoderma spp.)菌株,采用对峙法从中测定、筛选出一株对烟草赤星病菌(Alternaria alternata)具有极强拮抗作用的菌株(Tv－1),光学显微镜及透射电镜观察结果表明,Tv－1菌株的生防机制主要为生长竞争、重寄生作用以及产生生物活性类物质使病菌菌丝消解的直接抗菌作用,表现为Tv－1菌株菌丝生长迅速;能附着、缠绕于病菌菌丝上,并产生吸器侵入菌丝;同时还能分泌使病菌菌丝原生质浓缩、断裂、消解的物质。
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| 关 键 词： | 赤星病菌 绿色木霉 拮抗菌筛选 生防机制 |
| 文章编号： | 1004-390X(2000)03-0216-03 |
| 收稿时间： | 2000-4-18 |
Screening for the Isolates of Trichoderma viride Against Alten alternata and Its Biocontrol Mechanism |
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| Wang Ge,Zhou Xiaogang,Fang Dunhuang,Li Tianfei.Screening for the Isolates of Trichoderma viride Against Alten alternata and Its Biocontrol Mechanism[J].Journal of Yunnan Agricultural University,2000,15(3):216-218. |
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| Authors: | Wang Ge Zhou Xiaogang Fang Dunhuang Li Tianfei |
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| Institution: | Wang Ge
,Zhou Xiaogang
(Tobacco Research and Development Centre of Hongta Group, Yuxi 653100~na Group, Yuxi 653100)Fang Dunhuang
,Li Tianfei
(Yunnan Provincial Tobacco Research Istitute, Yuxi 653100) |
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| Abstract: | By the method of baiting, 18 isolates were obtained, and further identified as Trichoderma viride . The antagonistic tests of all these isolates to 7 strains of Alternaria alternata from diseased tobacco leaves from different area of Yunnan were carried out by pairing culture on PAD .The results showed that 1 isolate (Tv-1) was strong antagonistic. The examination of antagongonistic mechanism under optical microscope and transmission electron microscope showed Tv-1 hyphae growed quickly. lts hyphae could adhere to, coil around the hyphae of pathogen. Its finger-shaped haustorium penetrated into the hyphae of Alternaria alternata . Tv-1 could also secret some biologically active substance to make the pathogen hyphal plasma contracted, vacuolated and disrupted. |
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| Keywords: | Alternaria alternata Trichoderma viride Sceening of antagonistic organism Biocontrol mechanism |
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