聚乙二醇模拟干旱对甜高粱幼苗丙二醛、脯氨酸含量的影响*

 以甜高粱品种KFJT-CK及经过碳离子辐照选育出的早熟突变株KFJT-1为材料，用浓度分别为5%，10%和15%的聚乙二醇(PEG）6000模拟干旱对其进行胁迫处理，测定丙二醛(MDA）及脯氨酸(Pro)的含量。随着胁迫时间的延长和胁迫程度的增加，MDA含量持续升高; Pro含量在5%和10%PEG胁迫下持续升高，在15%PEG胁迫下先升高后降低。表明碳离子辐照可能使甜高粱膜脂过氧化特性发生改变，影响Pro的表达。为进一步研究碳离子束辐照对甜高粱的耐旱生理提供一定的基础，并为下一步的育种工作提供有用的参考。

Effects of Drought Stress Simulated by PEG on Malonaldehyde and Proline Contents of Sweet Sorghum Seedlings

The contents of malonaldehyde (MDA) and proline (Pro) were measured in seedlings of sweet sorghum KFJT CK and its premature mutant progeny KFJT-1 treated with 5%, 10% and 15% polyethylene glycol (PEG) 6000. After drought stress, MDA content in sweet sorghum seedling rapidly and persistently increased; the higher the PEG concentration was, the higher the MDA content was. Under 5% and 10% PEG stress, Pro content got more and more along with days and PEG concentration increase. However, under 15% PEG, Pro content firstly increased and then decreased. MDA contents had no apparent difference between KFJT-CK and KFJT-1 seedlings, but Pro content had remarkable difference between KFJT-CK and KFJT-1 seedlings. Carbon ion irradiation affected the peroxidation characteristics of membrane lipid and the Pro expression of sweet sorghum. This paper provides a foundation for further study on drought stressed physiology by carbon ion irradiation in sweet sorghum and some reference for the following breeding work.