| 稻飞虱基因组DNA提取方法的比较 |
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| 引用本文： | 刘佳妮,桂富荣,李正跃,沈文武.稻飞虱基因组DNA提取方法的比较[J].云南农业大学学报,2009,24(1):37-41. |
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| 作者姓名： | 刘佳妮 桂富荣 李正跃 沈文武 |
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| 作者单位： | 刘佳妮,桂富荣,李正跃,LIU Jia-ni,GUI Fu-rong,LI Zheng-yue(农业生物多样性与病虫害控制教育部重点实验室,生物多样性研究与应用技术国家工程中心,云南农业大学,植物保护学院,云南,昆明,650201);沈文武,SHEN Wen-wu(云南省建水县植保植检站,云南,建水,654300)
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| 基金项目： | 国家重点基础研究发展规划(973计划)，云南省科技攻关项目 |
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| 摘 要： | 以褐飞虱（Nilaparvata lugens Stal）为材料，分别采用SDS法，CTAB法，冰KAc法和饱和NaCl法提取褐飞虱的基因组DNA。通过电泳、紫外光谱分析和ISSR-PCR扩增等检测手段，比较分析了DNA数量与质量。结果表明： CTAB法和SDS法提取的DNA质量明显优于冰KAc法和饱和NaCl法，适于PCR扩增。并且SDS法提取的DNA经过PCR扩增后得到较多的多态性位点。因此，SDS法是实验室提取稻飞虱基因组有效，经济实用的方法。
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| 关 键 词： | 基因组DNA DNA提取 稻飞虱 |
| 收稿时间： | 2007-11-20 |
| 修稿时间： | 2007-12-21 |
Comparative Study on Methods for Extraction of Genomic DNA of Rice Planthopper |
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| LIU Jia-ni,GUI Fu-rong,LI Zheng-yue,SHEN Wen-wu.Comparative Study on Methods for Extraction of Genomic DNA of Rice Planthopper[J].Journal of Yunnan Agricultural University,2009,24(1):37-41. |
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| Authors: | LIU Jia-ni GUI Fu-rong LI Zheng-yue SHEN Wen-wu |
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| Institution: | 1Key Laboratory of Agro biodiversity and Pest Management of Education Ministry of China, The National Center for Agro biodiversity, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;2Plant Protect Station of Jianshui, Jianshui 654300, China |
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| Abstract: | Genomics DNA were extracted from the rice planthopper Nilaparvata lugens (Stal) using SDS, CTAB, KAc and NaCl method, respectively. DNA was detected by electrophresis, UV and ISSR PCR. The results suggested that, the quality of DNA samples extracted by CTAB method and SDS method was obviously better and more suitable for PCR than that by the other two methods, furthermore, SDS method presented the most polymorphic sites. Therefore, the SDS method was the most effective, practical and economic method in the genomic DNA extraction of planthopper in laboratory. |
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| Keywords: | genomic DNA DNA extraction rice planthopper |
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