| 冰核细菌冰核基因多态性的研究 |
| |
| 引用本文： | 姬广海,张世光.冰核细菌冰核基因多态性的研究[J].云南农业大学学报,1997,12(3):153-157. |
| |
| 作者姓名： | 姬广海 张世光 |
| |
| 作者单位： | 云南农业大学植物病理重点实验室 |
| |
| 基金项目： | 云南省自然科学应用基础科学基金 |
| |
| 摘 要： | 根据冰核细菌基因中的多拷贝重复编码区设计并合成简并引物inaZI5'-GCCGGTTATGGCAGC(T)ACGCTGACC-3'],通过聚合酶链式反应(Polymerase Chain Reaction,PCR)扩增20株不同种属的INA+和INA-细菌菌株.结果表明:所有供试的冰核活性菌株均获得扩增产物,而非冰核活性菌株则无扩增产物.冰核基因的扩增产物呈多态性.
|
| 关 键 词： | 冰核细菌 冰核基因 多态性 |
| 收稿时间： | 1996-12-25 |
Studies on Ice Nucleation Active Gene Diversity of Ice Nucleation Active Bacteria |
| |
| Ji Guanghai,Zhang Shiguang,Wu Jiangyu,Wei Jianfu,Chen Xinquan.Studies on Ice Nucleation Active Gene Diversity of Ice Nucleation Active Bacteria[J].Journal of Yunnan Agricultural University,1997,12(3):153-157. |
| |
| Authors: | Ji Guanghai Zhang Shiguang Wu Jiangyu Wei Jianfu Chen Xinquan |
| |
| Institution: | Department of Plant Pathology of Yunnan Agricultural University, Phytopathology Laboratory of Yunnan Province, Kunming 650201 |
| |
| Abstract: | According to the multicopy conservation of internal sequence repetion of ice nuleation active genes from ice nuclation active(INA)bactera, an oligonucleotide degeneracy primer inaZ I(5'GCCGGTTATGGCAGC(T)ACGCTGACC-3')was designed and synthesized with automatic DNA synthesizer Genes from more than 20 strains of different species and genus of INA+ and INA- bacteria were then amplified by polymerase chain reaction(PCR) The results showed that all the tested INA+ strains, except INA- strains, had their amplified products The amplified products of ice nuleation active gene are diversities. |
| |
| Keywords: | Ice nuleation active(INA) Ice nuleation active gene Diversity |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《云南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《云南农业大学学报》下载免费的PDF全文 |
