| RT－PCR方法检测番茄环斑病毒的研究 |
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| 引用本文： | 孔宝华,蔡 红,陈海如,沐咏民,唐 慧,刘进元.RT－PCR方法检测番茄环斑病毒的研究[J].云南农业大学学报,2001,16(2):96-98. |
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| 作者姓名： | 孔宝华 蔡 红 陈海如 沐咏民 唐 慧 刘进元 |
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| 作者单位： | [1]云南农业大学省植病重点实验室，云南昆明650201 [2]昆明动植物检疫避，云南昆明650034 |
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| 摘 要： | 根据番茄环斑病毒（TomRSV）外壳蛋白基因序列设计的引物P1,P2,用感病及健康组织总RNA为模板,进行RT-PCR试验,结果从感病组织中扩增出了470 bp的目的片段,而健康组织中无此扩增带。摸索各项实验条件,建立了RT-PCR检测番茄环斑病毒（TomRSV）的方法。
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| 关 键 词： | 番茄环斑病毒（TomRSV） RT-PCR 检测 |
| 收稿时间： | 2000-3-28 |
The Study on Detection Tomato Ring-spot Virus (TomRSV) by RT－PCR |
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| KONG Bao-hua,CAI Hong,CHEN Hai-ru,MU Yong-min,TANG Hui,LIU Jin-yuan.The Study on Detection Tomato Ring-spot Virus (TomRSV) by RT－PCR[J].Journal of Yunnan Agricultural University,2001,16(2):96-98. |
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| Authors: | KONG Bao-hua CAI Hong CHEN Hai-ru MU Yong-min TANG Hui LIU Jin-yuan |
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| Institution: | (1. Key Laboratory for Plant Pathology of Yunnan Province,Y A U,Kunming 650201,China;2. Kunming Animal and Plant Quarantine Bureau,Kunming 650034,China;3. Dept. of Biological Science and Biotechnology Tsinghua University,Beijing 100084,China) |
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| Abstract: | A pairs of primers of P1,P2 were designed,corresponding TomRSV RNA 2. The cDNA was made,and PCR was gotten on,with total RNA of the infected tissue and health tissue.The target fragment about 470bp was amplified from the infected sample, but not from health sample.More condition were tested, The method of detection of TomRSV by RT－PCR was built. |
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| Keywords: | Tomato Ring-spot Virus (TomRSV) RT－PCR Detection |
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