| 盾叶薯蓣组织培养技术研究* |
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| 引用本文： | 梁艳丽,李兰玉,谢世清.盾叶薯蓣组织培养技术研究*[J].云南农业大学学报,2006,21(2):164-168. |
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| 作者姓名： | 梁艳丽 李兰玉 谢世清 |
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| 作者单位： | 云南农业大学农学与生物技术学院,云南昆明650201 |
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| 基金项目： | 云南省应用基础研究项目 |
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| 摘 要： | 主要进行了盾叶薯蓣芽的诱导培养和带愈伤组织幼苗的生根培养。结果表明,芽诱导培养的17种培养基配方中,以MS+4mg/L BA+1mg/L KT为最好,其芽的诱导率达到68.72%;生根培养的4个培养基配方中,以MS+0.5mg/L NAA为最好,其生根率达到92.09%,且根粗、须根多。
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| 关 键 词： | 盾叶薯蓣 组织培养 芽诱导率 |
| 文章编号： | 1004-390X（2006）02-0164-05 |
| 收稿时间： | 2005-06-02 |
| 修稿时间： | 2005-06-02 |
Research on Tissue Culture Technology of Dioscorea zingiberensis |
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| LIANG Yan-li,LI Lan-yu,XIE Shi-qing.Research on Tissue Culture Technology of Dioscorea zingiberensis[J].Journal of Yunnan Agricultural University,2006,21(2):164-168. |
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| Authors: | LIANG Yan-li LI Lan-yu XIE Shi-qing |
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| Institution: | College of Agronomy and Biotechnology, Y A U, Kunming 650201, China |
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| Abstract: | Induction the differentiation of buds and roots of Dioscorea zingiberensis was studied in present paper. Results showed that MS medium with 4 mg/L BA and 1 mg/L KT was the best for the differentiation of buds. The bud induction rate was up to 68.72% and the buds were doing well. MS medium enriched with 0.5 mg/L NAA appeared to be the optimum medium for the differentiation of roots. The root induction rate was 92.09%. Furthermore, the roots inducted in the medium were thicker and more fibrous roots than in other 3 kinds of medium. |
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| Keywords: | Dioscorea zingiberensis tissue culture bud induction rate |
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