紫叶酢浆草鳞茎离体培养及快速繁殖研究

 采用紫叶酢浆草（Oxalis violalea）鳞茎作外植体源,进行离体培养及快速繁殖研究。结果表明:MS+6-BA 2.0 mg/L+NAA 0.5 mg/L诱导丛生芽发生,效果较好;丛生芽增殖用MS+6-BA 3.0 mg/L+NAA 0.5 mg/L每20 d转接1次增殖3～4倍。1/2 MS+NAA 2.5 mg/L+IBA 1.0 mg/L有利于壮苗和生根。从生根诱导到试管苗出瓶需20 d,生根率100%. 从丛芽增殖至生根出苗,整个培养周期只需40 d. 较已有报道的用叶片、叶柄组织培养的提前15～10 d,且繁殖速度极快,试管苗生长健壮,炼苗成活率达95%.

Research on Rapid in Vitro Propagation of Bulb of Oxalis violalea

Rapid in Vitro propagation of bulbs of Oxalis violalea was studied in this paper. The results showed as follows: the MS medium supplemented with 2.0 mg/L 6-BA+0.5 mg/L NAA was suitable for inducing axillary buds. MS +3.0 mg/L 6-BA+0.5 mg/L NAA was used for proliferation and the proliferation rate could reach 3～4 times in 20 day. 1/2 MS+2.0 mg/L NAA+1.0 mg/L IBA was suitable for rooting and getting strong plants. 20 d were needed from root induction to tube plant and the rooting rate could reach 100%. One cycle which was from proliferation to forming roots only needed 40 d. Bulb culture could be shortened for 10～15 d in contrast to culture of leafstalk and leaf which was reported before. The tube plants was very strong and the survival rate could reach to 95%.