| 滑叶藤ISSR PCR反应体系建立及优化 |
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| 引用本文： | 孙正海,王锦,李世峰,林开文,胡祎晨,王仕锦.滑叶藤ISSR PCR反应体系建立及优化[J].云南农业大学学报,2012,27(5):746-751. |
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| 作者姓名： | 孙正海 王锦 李世峰 林开文 胡祎晨 王仕锦 |
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| 作者单位： | 1.西南林业大学 园林学院，云南 昆明 650224；2.云南省农业科学院 花卉研究所，云南 昆明 650205 |
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| 摘 要： | 为建立和优化滑叶藤 ISSR PCR反应体系，运用正交试验和单因子试验分析模板DNA，TagDNA聚合酶，Primer，Mg2+和dNTPs 5个因子对滑叶藤ISSR PCR反应影响。结果表明了滑叶藤 ISSR PCR 25μL反应体系中5个因子最佳水平：DNA模板为84ng，TagDNA聚合酶为20u，Primer浓度为08mmol/L，dNTPs浓度为068mmol/L，M2+浓度为24mmol/L。该反应体系为利用ISSR分子标记技术研究铁线莲属植物提供了可靠方法。
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| 关 键 词： | 滑叶藤 ISSR PCR反应 体系优化 |
Establishment and Optimization of ISSR PCR Reaction System for Clematis fasciculifloora Franch |
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| SUN Zheng hai,WANG Jin,LI Shi feng,LIN Kai wen,HU Yi chen,WANG Shi jin.Establishment and Optimization of ISSR PCR Reaction System for Clematis fasciculifloora Franch[J].Journal of Yunnan Agricultural University,2012,27(5):746-751. |
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| Authors: | SUN Zheng hai WANG Jin LI Shi feng LIN Kai wen HU Yi chen WANG Shi jin |
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| Institution: | 1.School of Horticulture and Gardening, Southwest Forestry University, Kunming 650224，China; 2.Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205， China |
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| Abstract: | Effect for ISSR PCR reaction of Clematis fasciculifloora Franch on five factors （DNA template, TagDNA, primer, Mg2+ and dNTPs）were analyzed by orthogonal design and respective factor design for establishing and optimizing ISSR PCR reaction system of C fasciculifloora Franch As a result, the optimal ISSR PCR reaction system (25μL) mixture contained 84ng DNA template, 20u TagDNA, 08mmol/L primer, 24mmol/L Mg2+ and 068mmol/L dNTPs Theory sustaining for studying Clematis L plant by ISSR molecular marker were afforded from this experiment |
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| Keywords: | Clematis fasciculifloora Franch ISSR PCR reaction optimization of system |
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