| 云南龙陵黄山羊线粒体DNA限制性酶切分析 |
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| 引用本文： | 叶绍辉,王文.云南龙陵黄山羊线粒体DNA限制性酶切分析[J].云南农业大学学报,1999,14(1):54-57. |
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| 作者姓名： | 叶绍辉 王文 |
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| 作者单位： | [1]云南农业大学动物科学技术学院 [2]中国科学院昆明动物研究所 |
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| 基金项目： | 云南省重大应用基础研究基金 |
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| 摘 要： | 采用碱性变性法提取来自于龙陵县不同地区的18只黄山羊个体的线粒体DNA(mtDNA),并用ApaⅠ,AvaⅠ,BamHⅠ,BclⅠ,BglⅠ,BglⅡ,ClaⅠ,DraⅠ,EcoRⅠ,EcoRⅤ,HaeⅠ,HindⅢ,KpnⅠ,PstⅠ,PvuⅡ,SacⅠ,SalⅠ,SmaⅠ,StuⅠ和XhoⅠ等20种限制性内切酶进行酶切分析.结果发现龙陵黄山羊线粒体DNA的分子量大小约为15.8Kb;不同酶的酶切位点分别为:DraⅠ有7个酶切位点,AvaⅡ有6个酶切位点,EcoRⅤ和StuⅠ共有5个酶切位点,HindⅢ和HeaⅡ有4个酶切位点,BamHⅠ,BglⅡ,PstⅠ和PvuⅡ有3个酶切位点,ApaⅠ,ClaⅠ有2个酶切位点,其余有1个酶切位点.
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| 关 键 词： | 龙陵黄山羊 线粒体DNA(mtDNA) 限制性酶切类型 |
| 收稿时间： | 1997-03-15 |
Restriction Cleavage Analysis of mtDNA from Yunnan Red Goat |
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| Ye ShaohuiPeng,HeluWang WenLiu Aihua.Restriction Cleavage Analysis of mtDNA from Yunnan Red Goat[J].Journal of Yunnan Agricultural University,1999,14(1):54-57. |
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| Authors: | Ye ShaohuiPeng HeluWang WenLiu Aihua |
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| Institution: | Ye Shaohui1Peng
Helu1Wang Wen2Liu Aihua2 |
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| Abstract: | Mitochondral DNAs of Yunnan red goat distributed in the different regions of Longling county of
Yunnan province has been digested with twenty restriction endonucleases,i.e.:Apa ,Ava ,BamH
,Bcl ,Bgl ,Bgl ,Cla ,Dra ,EcoR ,EcoR ,Hae ,Hind ,Kpn ,Pst ,Pvu ,Sac ,Sal ,Sma ,Stu and Xho .The
molecular weight of the fragements is about 158kb.mtDNAs were broken into different sizes by
the different restriction endonucleases .There are 7 fragments for Dra ,6 for Ava ,5 for Ecor and
Stu ,4 for Hind and Hae ,3 for BamH ,Bgl ,Pst and Pvu ,2 for Apa and Cla ,and 1 for the
otheres. |
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| Keywords: | Yunnan Red Goat mtDNA Restriction cleavage pattern HJ] |
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