| 蜡蚧轮枝菌昆明菌株(KM9803)的生物学特性研究(二)——培养基种类、储藏条件对菌落生长、产孢量和孢子萌发率的影响* |
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| 引用本文： | 徐力文,周天雄,杨美林.蜡蚧轮枝菌昆明菌株(KM9803)的生物学特性研究(二)——培养基种类、储藏条件对菌落生长、产孢量和孢子萌发率的影响*[J].云南农业大学学报,2006,21(2):175-177. |
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| 作者姓名： | 徐力文 周天雄 杨美林 |
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| 作者单位： | 徐力文（云南农业大学植物保护学院,云南,昆明,650201）
周天雄（云南农业大学植物保护学院,云南,昆明,650201）
杨美林（云南农业大学植物保护学院,云南,昆明,650201） |
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| 摘 要： | 用7种培养基培养的结果,在麦芽汁培养基(MEA)上(23℃)得到最大产孢量(4.7×109分生孢子/皿);用6种谷物的固体培养基试验中,大米粒培养基最适宜产孢,发酵培养10d得到最大产孢量1.5×109分生孢子/g,孢子萌发率95.14% 。3种储藏条件试验的结果,以4℃冰箱中黑暗下6个月的孢子萌发率最高(91.47%)。
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| 关 键 词： | 培养基 储藏条件 菌落 分生孢子 产孢量 萌发率 |
| 文章编号： | 1004-390X（2006）02-0175-03 |
| 收稿时间： | 2005-08-30 |
| 修稿时间： | 2005-08-30 |
A Study of Biological Character of Verticillium lecanii KM9803 Strain Isolated from Kunming(Ⅱ)--The Effects of Media Kinds and Preserved Condition on Colonial Growth、Conidiospore Yield and Conidia Germination Rata |
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| XU Li-wen,ZHOU Tian-xiong,YANG Mei-lin.A Study of Biological Character of Verticillium lecanii KM9803 Strain Isolated from Kunming(Ⅱ)--The Effects of Media Kinds and Preserved Condition on Colonial Growth、Conidiospore Yield and Conidia Germination Rata[J].Journal of Yunnan Agricultural University,2006,21(2):175-177. |
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| Authors: | XU Li-wen ZHOU Tian-xiong YANG Mei-lin |
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| Institution: | (College of Plant Protection, Yunnan Agricultural University,Kunming 650201,China) |
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| Abstract: | The maximum sporulation yield (4.7×109 conidia/dish)was obtained on MEA media at 23℃.Experiments of culturing the fungus on six solids grain media showed the rice grain was the most suitable for sporulation yield,as 1.5×109 conidia/g fermented for 10 days.The result of experiments on three kinds preserved condition showed the germination rate of conidiospore was 91.4% in 4℃ refrigerator after 6 months. |
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| Keywords: | media preserved condition colonial growth conidiospore yield conidia germination |
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