烟草黑胫病菌分子检测

随着聚合酶链式反应 (ＰＣＲ)技术的发展 ,用特异引物进行真菌种间核糖体ＤＮＡ片段进行ＰＣＲ扩增以达到鉴定生物种的目的已成为一种简便、快捷的技术和方法。本试验根据从ＧｅｎｅＢａｎｋｄａｔａｂａｓｅ获得的疫霉属真菌 (Ｐｈｙｔｏｐｈｔｈｏｒａｓｐｐ .)ｒＤＮＡＩＴＳ区段序列 ,合成了 1对 1 7ｂｐ特异寡聚核苷酸引物进行了不同病原菌、病组织及土壤中寄生疫霉菌 (Ｐａｒａｓｉｔｉｃａｖａｒ.ｎｉｃｏｔｉａｎａｅ)的特异扩增试验 ,提供并完善了一套快速检测该菌的技术和方法。1　材料与方法1 1　供试菌株　烟草疫霉菌菌株 ,交…

Molecular Detection of Phytophthora parasitica Var. Nicotianae From Diseased Plants and Soil

Statistics comparing and analysis were done on students in Y A U including their 5 items of body shape and physiological function index. Some scientific basis for physical culture teaching and competition were put forward. Key words Figure; Physiological function; Constitution into three stages At the second stage, COD was removed by 8 3%,NH 3 N by 79 1% At the third stage, COD was removed by 41 9%,NH 3 N by 68 5%,TP by 85 4%,and the color by 84 4% Comparing two processes, it was indicated that the purification effect of fixing algae process on anaerobic digested slurry from pig waste was significantly better than that of floating algae Besides, during the purification, a large quantity of mixed algae could be produced,which could be used as the additive of green feed for breeding various economic animals,and increase the quality and quantity of animals