观赏凤梨再生体系的建立及优化*

 以5个品种（系）观赏凤梨的腋芽为外植体，研究了不同基因型、激素配比和苗龄对芽分化的影响。采用L9(34)正交试验分别针对TDZ，6-BA，NAA，IBA，蔗糖、大量元素及椰乳（CW）等因素进行优化，筛选出最佳的增殖培养基和生根培养基。结果表明：芽分化的最佳培养基为MS+TDZ 1.5mg/L +6-BA 0.5mg/L。在此分化培养基上，紫星和红星的芽分化频率较高，达76.8%，70.6%。黄星、火炬和红剑芽分化频率较低，分别为44.2%，27.4%和 24.9%。7d苗龄的腋芽外植体最适于再分化。最佳增殖培养基为MS + TDZ 0.5mg/L + 6-BA 1.0mg/L +NAA 0.2mg/L+蔗糖40g/L，其增殖系数达7.31。最佳的生根培养基为1/2MS + NAA 0.5 mg/L + IBA 1.0mg/L +CW 20mL/L，生根率达60%以上

Establishment and Optimization of Regeneration System of Ornamental Bromeliads

Five ornamental bromeliads varieties were used to investigate the effects of different genotypes, hormone combinations and seedling ages on shoot differentiation Based the recipe, the effects of TDZ,6-BA,NAA,IBA,sucrose,MS and coconut water (CW) were investigated to optimize the shoot regeneration system throughL9(34)orthogonal experiment and to select the optimal rooting medium The results showed that the medium optimal for adventitious buds was MS+TDZ 1.5mg/L+6-BA 0.5mg/L. In this differentiation medium, different ornamental bromeliads varieties differed greatly in their regeneration capacities, Zixing and Hongxing were 76.8% and 70.6%,respectively, but Huangxing, Huoju and Hongjian were 44.2%,27.4% and 24.9%,respectivelyThe explants for 7-day-old seedling had a high differentiation rateThe optimal medium for the propagation of adventitious buds consists of MS +TDZ 0.5mg/L + 6-BA 1.0mg/L +NAA 0.2mg/L +sucrose 40g/L, the multiplication coefficient reached to 7.31; and the optimal medium for the rooting was 1/2MS +NAA 0.5mg/L + IBA 1.0mg/L +coconut water extract 20mL/L Rooting rate was over 60%