玉米小斑病菌诱导大豆叶片胼胝质沉积的初步研究

 利用菌丝染色和胼胝质特异性染色技术，研究玉米小斑病菌(Bipolaris maydis)与非寄主植物大豆互作时，能否诱导大豆产生胼胝质沉积相关的防御反应。将玉米小斑病菌接种到大豆叶片3h后即可观察到孢子能在大豆叶片上萌发，12h后能诱导大豆叶片细胞中胼胝质沉积。利用实时荧光定量PCR技术，在转录水平检测到β-1,3-葡聚糖酶基因（GLU）表达有变化。本试验研究结果表明大豆作为玉米小斑病菌的非寄主植物，可受玉米小斑病菌诱导而产生胼胝质沉积的防御反应，为进一步探明作物多样性种植降低病害发生的非寄主抗性机理奠定基础。

Callose Deposition in the Leaf Cells Associated with InducedResistance of Glycine max to Bipolaris maydis

To learn the role of induced resistance of non host happened in the cultivation system of crop diversity, Glycine max were inoculated with the non host pathogen of Bipolaris maydis. After 3 hours inoculation, B. maydis could germinate hyphae on the G. max leaves, and then infected the non host plant G. max by fungi staining technique with aniline blue, and callose deposition could be observed after 12 hours of inoculation. In addition, the results of real time fluorescence quantitative PCR indicated that up and down regulated expression of β-1, 3-glucanases gene (GLU) happened within 3 and 72 hours after inoculation. The results suggested that G. max could be induced to produce callose deposited in the cell for resistance reaction when inoculated with B. maydis, which could partly explain molecular mechanism of non host resistance of biodiversity for sustainable crop disease management.