| 甘蔗宿根矮化病的TBIA快速检测* |
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| 引用本文： | 李文凤,黄应昆,王哓燕,吴才文,罗志明,陈学宽,卢文洁.甘蔗宿根矮化病的TBIA快速检测*[J].云南农业大学学报,2010,25(1):132-135. |
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| 作者姓名： | 李文凤 黄应昆 王哓燕 吴才文 罗志明 陈学宽 卢文洁 |
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| 作者单位： | 云南省农业科学院 甘蔗研究所，云南省甘蔗遗传改良重点实验室，云南 开远 661600 |
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| 摘 要： | 利用从澳大利亚引进的RSD标准抗原抗体，研究建立了TBIA快速检测甘蔗宿根矮化病（RSD）的方法。通过对田间采集的样本进行检测，并用I-ELISA检测法验证，结果表明：TBIA能简便、快速、准确、有效的检测出RSD，且检测效率高，适合于田间大批量样品快速检测。该方法为甘蔗宿根矮化病的诊断和防治、脱毒种苗的生产、对外甘蔗品种/材料交换检疫检测提供了技术支撑。
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| 关 键 词： | 甘蔗宿根矮化病 组织斑点免疫TBIA 检测 |
| 收稿时间： | 2008-9-1 |
| 修稿时间： | 2008-10-3 |
Rapid Detection of Sugarcane Ratoon Stunting Disease by TBIA |
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| LI Wen-feng,HUANG Ying-kun,WANG Xiao-yan,WU Cai-wen,LUO Zhi-ming,CHEN Xue-kuan,LU Wen-jie.Rapid Detection of Sugarcane Ratoon Stunting Disease by TBIA[J].Journal of Yunnan Agricultural University,2010,25(1):132-135. |
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| Authors: | LI Wen-feng HUANG Ying-kun WANG Xiao-yan WU Cai-wen LUO Zhi-ming CHEN Xue-kuan LU Wen-jie |
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| Institution: | Research Institute of Sugarcane，Yunnan Academy of Agricultural Sciences，Key Laboratory for Sugarcane Improvement of Yunnan Province,Kaiyuan 661600，China |
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| Abstract: | The detecting method of TBIA for RSD was established based on standard antigen and antibody from AustraliaThe results indicated that RSD could be conveniently，rapidly，exactly and effectively detected by TBIA for samples collected from field，and the detected results were consistent with that of I ELISA detectionSo TBIA could be used in RSD diagnosis and control，disease free sugarcane seedling production，and quarantine and detection of sugarcane materials exchanged with external |
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| Keywords: | sugarcane ratoon stunting disease tissue blot immunoassay detection |
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