不同浓度6-BA和AgNO3对甘蓝叶片不定芽分化的影响

 探索不同浓度的6 BA和AgNO3对甘蓝叶片分化的影响，以期为甘蓝基因工程育种，尤其是质体基因工程育种奠定基础。试验以甘蓝叶片作为外植体，研究了不同浓度的6 BA，AgNO3对甘蓝叶片芽分化的影响。结果表明：在MS+6BA1mg/L+AgNO3 0.01mg/L的培养基上芽的诱导率最高，达到96.7%。增殖率最高的培养基为MS+6BA 1mg/L+AgNO3 0.05mg/L，达到7.99。叶片高效离体再生体系的建立，为甘蓝叶绿体基因转化打下了一个良好基础。

Effect of 6 BA and AgNO3 on Shoot Regeneration of Cabbage Leaf

Transplastomic transformation has become attractive technology in plant molecular breeding. However, the efficiency of plasmid transformation limited because of low regeneration in many plants. AgNO3 as a competitive inhibitor regulating the synthesis of ethylene and polyamine plays a very important role in organogenesis or morphogenesis of in vitro cultures. The research was conducted to study the effects of different concentrations of 6 benzylaminopurine（6 BA）and AgNO3 on inducing adventitious buds from cabbage (Brassica oleracea var. capitata L.) leaf. In the experiment, cabbage leaf of aseptic seedling was cut into segments measured about 0.5cm×0.5cm, cultured on the MS medium supplemented with different concentrations of 6 BA and AgNO3. The results showed that the optimal induction medium was MS+6 BA 1mg/L+AgNO3 0.01mg/L, the inducing rate reached as high as 96.7%. While the optimal proliferation medium was MS+6 BA 1mg/L+AgNO30.05mg/L, proliferation rate was 7.99. A high efficiency regeneration system of leaf explants in cabbage has been developed based on the study, which lay the foundation for further research of cabbage chloroplast transformation.