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基于线粒体DNA控制区(mtDNA Dloop)序列分析瓢鸡的遗传多样性
引用本文:贡潘偏抽,刘丽仙,李大林,袁跃云,孙利民,杨润,刘文瑜,刘伟,苗永旺.基于线粒体DNA控制区(mtDNA Dloop)序列分析瓢鸡的遗传多样性[J].云南农业大学学报,2011,26(2):211-214.
作者姓名:贡潘偏抽  刘丽仙  李大林  袁跃云  孙利民  杨润  刘文瑜  刘伟  苗永旺
作者单位:1云南农业大学 动物科学技术学院,云南 昆明 650201;2云南农业职业技术学院 畜牧兽医系,云南 昆明 650212;3云南省家畜改良工作站, 云南 昆明 650021; 4云南省普洱市畜牧工作站, 云南 普洱 665000;5云南省普洱市镇沅县畜牧工作站,云南 镇沅 666500
摘    要: 采用PCR产物直接测序技术对云南省镇沅县50只瓢鸡样品的线粒体DNA控制区(mtDNA D loop)第Ⅰ高变区序列进行了分析。结果表明:在所分析的Dloop区序列中(520bp),T,C,A,G平均含量分别为30.4%,29.6%,27.4%,12.6%;共检测到27个核苷酸多态位点,其中24个为转换,3个为颠换,序列突变率为5.19 %;序列存在13种单倍型,单倍型多样度(H)为0.883±0.028,核苷酸多样度(π值) 为0.01503±0.00099,表明瓢鸡群体内遗传变异较丰富。瓢鸡样品可划分为A,B,E,F,G等5个世系,在NJ无根系统树上聚为5大枝,揭示瓢鸡在遗传组成上具有5个母系血统来源。

关 键 词:瓢鸡  线粒体DNA  D  loop  单倍型  遗传变异  核苷酸多样度

The Investigation of Genetic Diversity of Piao Chicken Based on Mitochondrial DNA D loop Region Sequence
GONGPAN Pian-chou,LIU Li-xian,LI Da-lin,YUAN Yue-yun,SUN Li-min,YANG Run,LIU Wen-yu,Liu Wei,MIAO Yong-wang.The Investigation of Genetic Diversity of Piao Chicken Based on Mitochondrial DNA D loop Region Sequence[J].Journal of Yunnan Agricultural University,2011,26(2):211-214.
Authors:GONGPAN Pian-chou  LIU Li-xian  LI Da-lin  YUAN Yue-yun  SUN Li-min  YANG Run  LIU Wen-yu  Liu Wei  MIAO Yong-wang
Institution:1 College of Animal Science and Technology, Agricultural University,Kunming 650201, China; 2 Department of Animal Husbandry and Veterinary medicine, Yunnan Agricultural College of Vocational Education, Kunming  650212,China;3 Domestic Animal Breeding and Crossbreed improvement Station of Yunnan Province,Kunming 650021, China; 4 Animal Husbandry Station of Puer City, Puer 665000, China;5 Animal Husbandry Station of Zhenyuan County,Zhenyuan 666500, China
Abstract:In the present paper, the PCR products of the mitochondrial D loop sequences (520 bp in length) of 50 individuals from Piao chicken (which have no pygostyle) of Zhenyuan County, Yunnan province, China, were directly sequenced. The results revealed that the average percentage values of base composition of T, C, A and G in the sequences were 30.4%,29.6%,27.4% and 12.6%, respectively. In the samples assayed, 27 mutation sites included 24 transitions and 3 transversions viz 5.19% mutation rate and 13 haplotypes were found, haplotype diversity (H) was 0.883±0.028 and nucleotide diversity (π value) was 0.01503±0.00099.Phylogenetic analysis by using the former study and the unrooted NJ method showed that all the sequences were clustered in 5 major clades. The results in this study indicated that the genetic variability in Piao chicken population was high and there were 5 maternal origins to this chicken.
Keywords:Piao chicken  mtDNA D loop region  haplotype  genetic variability  nucleotide diversity
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