| 玉米SSR与盐溶蛋白指纹图谱分析 |
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| 引用本文： | 聂琼,徐如宏,柏光晓,张文龙,姜军伟,赵波.玉米SSR与盐溶蛋白指纹图谱分析[J].贵州大学学报(农业与生物科学版),2006,25(6):471-474,535. |
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| 作者姓名： | 聂琼 徐如宏 柏光晓 张文龙 姜军伟 赵波 |
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| 作者单位： | 1. 贵州大学,农学院,贵州,贵阳,550025
2. 贵州省种子站,贵州,贵阳,550001 |
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| 摘 要： | 利用SSR标记法与盐溶蛋白法对32份玉米自交系和13个杂交种的指纹图谱进行分析，结果表明，盐溶蛋白图谱中共有35条不同的谱带，多态性信息量占74．29％。筛选了50个SSR引物，其中6个多态性好的SSR引物能区分所有供试材料。在32个自交系中共检测到29个等位基因，平均每个引物4．83个，多态性信息量占93．10％。仅用2个引物就检测到10个位点，把13个杂交种分开，并构建了13个杂交种的数字指纹图谱。
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| 关 键 词： | 玉米 盐溶蛋白 SSR标记 指纹图谱 |
| 文章编号： | 1008-0457（2006）06-0471-04 |
| 收稿时间： | 2006-09-06 |
| 修稿时间： | 2006-09-06 |
Analysis of SSR and salt-soluble protein fingerprinting in maize |
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| NIE Qiong,XU Ru-hong,BAI Guang-xiao,ZHANG Wen-long,JIANG Jun-wei,ZHAO Bo.Analysis of SSR and salt-soluble protein fingerprinting in maize[J].Journal of Mountain Agriculture & Biology,2006,25(6):471-474,535. |
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| Authors: | NIE Qiong XU Ru-hong BAI Guang-xiao ZHANG Wen-long JIANG Jun-wei ZHAO Bo |
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| Institution: | 1. College of Agriculture, Guizhou University, Guizhou Guiyang 550025,China; 2. Guizhou Provincial Seed Management Office, Guizhou Guiyang 550001, China |
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| Abstract: | SSR and salt-soluble protein fingerprinting in 32 maize inbred lines and 13 hybrids were analysed. The results showed that 35 different salt-soluble protein bands were detected and the polymorphism information was 74.29%. 50 primers were used in SSR analysis and only 6 high polymorphism primer combinations could distinguish all the hybrid and inbred lines. 29 alleles were detected with 6 SSR primers in 32 maize inbred lines, the average number of alleles of each loci was 4. 83. The polymorphism information was 93. 10%. Only 2 primer combinations could distinguish 13 hybrid,29 alleles were detected and build the SSR fingerprint of 13 hybrids. |
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| Keywords: | maize salt-soluble protein SSR maker fingerprinting |
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