| 鸭肝炎病毒RT-PCR检测方法的建立与应用 |
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| 引用本文： | 鲜思美,汪德生,龙运桃.鸭肝炎病毒RT-PCR检测方法的建立与应用[J].贵州大学学报(农业与生物科学版),2012,31(5):403-405. |
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| 作者姓名： | 鲜思美 汪德生 龙运桃 |
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| 作者单位： | 1. 贵州大学动物科学学院,贵州贵阳550025;贵州大学贵州省动物疫病研究所,贵州贵阳550025
2. 贵州大学动物科学学院,贵州贵阳,550025 |
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| 摘 要： | 根据鸭肝炎病毒(Duck Hepatitis Virus,DHV)3D基因序列,设计合成1对引物,通过优化RT-PCR反应条件,建立了DHV的RT-PCR检测方法。特异性试验结果显示,该引物仅特异性扩增出DHV 460 bp的特异性片段,而扩增鸭瘟病毒(DPV)、鹅细小病毒(GPV)、番鸭细小病毒(MDPV)和鹅副粘病毒(GPMV)的核酸扩增结果均为阴性;敏感性试验结果显示,该方法能检测到100 pg的DHV核酸;对6份临床病料进行RT-PCR扩增,DHV的检出率为83.33%(5/6)。结果表明:该方法具有良好的特异性和敏感性,可对临床病料中的DHV进行快速检测。
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| 关 键 词： | 鸭肝炎病毒 RT-PCR 特异性 敏感性 |
Establishment of RT-PCR Assay for Duck Hepatitis Viruses |
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| XIAN Si-mei
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WANG De-sheng
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LONG Yun-tao.Establishment of RT-PCR Assay for Duck Hepatitis Viruses[J].Journal of Mountain Agriculture & Biology,2012,31(5):403-405. |
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| Authors: | XIAN Si-mei WANG De-sheng LONG Yun-tao |
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| Institution: | 1. College of Animal Science, Guizhou University, Guiyang Guizhou 550025, China; 2. Institute of Animal Disease of Guizhou Province, Guizhou University, Guiyang Guizhou 550025, China) |
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| Abstract: | A pair of specific primers were designed and synthesized according to 3D gene sequence of Duck Hepatitis Virus （DHV）. After optimization of RT-PCR conditions, a RT-PCR assay for detecting the genomic RNA of DHV was developed. The results revealed that the expected 460 bp fragment could be scored from the extracted DHV RNA by the RT-PCR assay. The RT-PCR products couldn＇ t been obtained from samples con- taining duck plague virus（DPV） , Muscovy duck parvovirus（ DPV ）, Goose parvovirus（GPV） and Goose pa- rarnyxovirus （GPMV）. The PCR was more sensitive by this experiment, and would be applied for rapid diag- nosis of DHV infection since the minimum detection amount was as low as 100 pg. |
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| Keywords: | Duck Hepatitis Virus RT-PCR specificity sensitivity |
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