| 博落回生物碱提取工艺研究 |
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| 引用本文： | 王珂佳,谭艾娟,刘佳穆.博落回生物碱提取工艺研究[J].贵州大学学报(农业与生物科学版),2010,29(1):52-55,66. |
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| 作者姓名： | 王珂佳 谭艾娟 刘佳穆 |
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| 作者单位： | 贵州大学,生命科学学院,贵州,贵阳,550025 |
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| 基金项目： | 贵州省国际科技合作项目 |
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| 摘 要： | 以博落回血根碱的提取率为考察指标优化博落回生物碱提取工艺,结果表明:酸性醇提取法比酸水法、醇提法对血根碱的提取效率高。采用正交试验方法对酸性醇提取法进行优化,最佳工艺条件为:在pH值为3的75%乙醇溶液中回流提取2次,每次1 h。此条件下血根碱提取率为5.82 mg/g。对最佳工艺条件稳定性试验的结果表明:其稳定性较好,RSD=1.65%。
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| 关 键 词： | 博落回 酸性醇 生物碱 血根碱 正交试验 |
An Extraction Procedure of Alkaloid from Macleay Acordata |
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| WANG Ke-jia,TAN Ai-juan,LIU Jia-mu.An Extraction Procedure of Alkaloid from Macleay Acordata[J].Journal of Mountain Agriculture & Biology,2010,29(1):52-55,66. |
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| Authors: | WANG Ke-jia TAN Ai-juan LIU Jia-mu |
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| Institution: | ( College of Life Sciences, Guizhou University, Guiyang 550025,China) |
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| Abstract: | Based on the extraction rate index of Alkaloid, the extraction method of Alkaloid from Macleay Acordata was optimized in the current work. The results indicated that among the tested methods, the extraction protocol using acidic alcohol solution was the best. Optimal extraction conditions of this method were investigated by orthogonal design. It was revealed that the extraction rate of Sanguinarine was up to 5.82 mg/g when the condition using 75 % ethanol solution （pH = 3.0） and extracting two times for 1 h ed. The extraction rate of Sanguinarine in repeat experiments under the optimal conditions stability （ RSD = 1.65% ）. |
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| Keywords: | Macleaya Cordata Acid Alcohol alkaloi Sanguinarine orthogonal design each was adopt- was considerably |
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