| 红曲霉中麦角固醇皂化条件的优化 |
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| 引用本文： | 张丽丽,谭艾娟,刘爱英,刘芸,吕世明.红曲霉中麦角固醇皂化条件的优化[J].贵州大学学报(农业与生物科学版),2010,29(2):143-146. |
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| 作者姓名： | 张丽丽 谭艾娟 刘爱英 刘芸 吕世明 |
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| 作者单位： | 1. 贵州大学,生命科学学院,贵州,贵阳,550025
2. 贵州大学,动物科学学院,贵州,贵阳,550025 |
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| 摘 要： | 应用单因子和正交两种优化方法,考察红曲霉Monascus菌株-ZZ产麦角固醇皂化的最佳条件。单因子实验结果表明:最佳皂化条件碱体积分数为20%,醇碱比为2∶1,物料与碱的比例为1∶1,皂化时间为2 h,皂化温度为90℃。正交优化结果显示:不同因子对麦角固醇提取的影响顺序是皂化温度皂化时间物料皂化剂比碱体积分数醇碱比;最佳条件是物料皂化剂比为1∶1,碱体积分数为15%,醇碱比为4∶1,皂化温度为90℃,皂化时间为3 h。
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| 关 键 词： | 红曲霉 麦角固醇 皂化 正交优化 |
Optimization of Ergosterol Saponification Conditions in Monascus Mycelium |
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| ZHANG Li-li,TAN Ai-juan,LIU Ai-ying,LIU Yun,L Shi-ming.Optimization of Ergosterol Saponification Conditions in Monascus Mycelium[J].Journal of Mountain Agriculture & Biology,2010,29(2):143-146. |
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| Authors: | ZHANG Li-li TAN Ai-juan LIU Ai-ying LIU Yun L Shi-ming |
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| Institution: | ZHANG Li-li,TAN Ai-juan,LIU Ai-ying,LIU Yun,L(U) Shi-ming |
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| Abstract: | The single factor test and orthogonal experiments had been carried out to optimize the condition of ergosterol saponification from the Monascus sp. ZZ mycelium. The result of single factor test indicated that the optimal conditions were 20% of caustic soda concentration, 2：1 of ethanol to alkali, 1 ： 1 of sample to the alkali, and saponification at 90℃ for 2 h. Orthogonal test showed that the effect order among the factors were the saponified temperature 〉 the saponified time 〉 the ratio of sample to saponifier 〉 the caustic soda concentration 〉 the ratio of ethanol to alkali. The optimal ratio of sample to saponifier, caustic soda concentraratio of ethanol to alkali, saponified temperature, and saponified time are 1： 1, 15%, 4： 1,90℃, 3 h,respectively. |
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| Keywords: | Monascus Ergosterol Saponification Optimization |
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