| Box-Behnken设计优化贵州野生地枇杷总黄酮提取工艺 |
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| 引用本文： | 陈琳,俸婷婷,游婵,李超.Box-Behnken设计优化贵州野生地枇杷总黄酮提取工艺[J].贵州大学学报(农业与生物科学版),2016(4):83-87. |
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| 作者姓名： | 陈琳 俸婷婷 游婵 李超 |
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| 作者单位： | 1. 贵州大学 药学院，贵州 贵阳550025; 贵州省药食同源植物资源开发研究开发中心，贵州 贵阳550025; 贵州省药食两用资源应用开发工程实验室，贵州 贵阳550025;2. 贵州大学 药学院,贵州 贵阳,550025 |
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| 基金项目： | 贵州省科学技术基金项目(黔科合J字[2012]2173号);贵州省科技合作计划项目(黔科合LH字[2015]7666号);贵州省药食同源资源研究开发科技创新人才团队(黔科合人才团队(2015)4010号);贵州省药食同源植物资源开发工程技术研究中心(黔科合G字(2015)4001);贵州省药食两用资源应用开发工程实验室(黔发改投资[2015]542号);贵州省中药民族药制药工程专业学位研究生工作站(黔教研合JYSZ字[2014]002) |
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| 摘 要： | 在各单因素实验基础上,以总黄酮提取率为指标,通过Box-Behnken响应面设计考察乙醇浓度、料液比、提取时间、提取次数对地枇杷总黄酮提取工艺的影响,优化贵州野生地枇杷总黄酮提取工艺。结果表明:地枇杷总黄酮的乙醇回流最佳提取工艺条件的酒精浓度为70%,液固比1∶15,提取时间为30 min,提取次数为3次,地枇杷总黄酮提取率为5.09%。按最优提取条件进行3次验证实验,最终地枇杷总黄酮平均提取率5.13%。本文利用乙醇回流提取地枇杷总黄酮工艺操作简便,稳定可行。
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| 关 键 词： | 地枇杷 乙醇回流提取 Box-Behnken设计 |
Optimization of Extracting Total Flavonoids in Wild Ficus tikoua by Box-Behnken Design |
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| CHEN Lin;FENG Ting-ting;YOU Chan;LI Chao.Optimization of Extracting Total Flavonoids in Wild Ficus tikoua by Box-Behnken Design[J].Journal of Mountain Agriculture & Biology,2016(4):83-87. |
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| Authors: | CHEN Lin;FENG Ting-ting;YOU Chan;LI Chao |
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| Institution: | CHEN Lin;FENG Ting-ting;YOU Chan;LI Chao;School of Pharmaceutical Sciences,Guizhou University;Medicinal and Edible Plant Resources Research and Development Center of Guizhou;Medicinal and Edible Plant Resources Application and Development Engineer Laboratory of Guizhou; |
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| Abstract: | In this research we tested the effects of ethanol concentration, solid-liquid ratio, time and period of extraction on extraction rateof total flavonoids from F.tikoua by Box-Behnken design to optimize extracting technology based on sin-gle factor test. The results showed that, the optimum ethanol reflux extraction conditions of total flavonoids in F.tikouawas as follow:extracting 3 times with the alcohol concentration of 70%, liquid-solid ratio of 15, 30 min each time.The yield of total flavonoids was 5.09%. The optimum extraction conditions wasverifiedforthree times, and the average yield of total flavonoids was 5.13%. Extracting total flavonoids from wild F.tikoua by ethanol reflux process is a simple, stable and fea-sible method. |
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| Keywords: | Ficustikoua Ethanolreflux extraction Box-Behnken design |
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