适于悬浮培养的半夏疏松愈伤组织诱导及增殖技术

用半夏叶片或叶柄作外植体,通过调节植物生长调节物质的种类、浓度及培养条件,以获得适于悬浮培养的半夏疏松愈伤组织。结果表明,添加2,4-D较NAA诱导的愈伤组织疏松,将叶片接种于MS+2,4-D 1.0 mg/L+6-BA 1.5 mg/L+蔗糖30 g/L+琼脂6 g/L或叶柄接种于MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂6 g/L上诱导出的愈伤组织长势较好且疏松,诱导率分别达到100%和95.6%,愈伤组织鲜重分别为0.932 g和0.622 g。将上述愈伤组织以MS+2,4-D 2.0 mg/L+6-BA 1.0 mg/L+蔗糖30 g/L+琼脂6 g/L培养基连续继代3次,便获得适于建立细胞悬浮培养体系的疏松愈伤组织,从而为半夏人工种子生产必须的同步化高质量人工种胚的培养提供可靠材料。

Induction andmultiplication of loose callus of Pinellia ternata [ Alismatales:Araceae} suitable for suspension cul￣ture

Using Pinellia ternata leaf or petiole as explants to obtain loose callus suitable for suspension culture by adjus﹣ting the concentrations of different plant growth regulators and the culture conditions. The results showed that the callus was looser in the media containing 2,4-D than that containing NAA. The induced callus grew better and looser when leav﹣es were inoculated on the medium made of MS+ 2,4-D 1.0 mg/L+ 6-BA 1.5 mg/L+sucrose 30 g/L+agar 6 g/L, or petioles were inoculated on the medium consisted of MS+ 2,4-D 1.0 mg/L+ 6-BA 1.0 mg/L+sucrose 30 g/L-1+agar 6 g/L. The induction rates were 100% and 95.6%, respectively, and the callus fresh weights were 0.932 g and 0.622 g, respectively. After these calluses were continuously cultured three more times in the medium of MS+ 2,4-D 2.0 mg/L+6-BA 1.0 mg/L + sucrose 30 g/L + agar 6 g/L, the loose calluses were suitable for establishing cell suspension culture system. These loose calluses become the reliable materials for the necessary synchronization of artificial seeds for high quality production of artificial embryo culture of Pinellia ternata .