首乌藤不同部位有效成分含量考察

目的:考察并比较首乌藤嫩、中、老枝不同部位中主要有效成分二苯乙烯苷、大黄素、大黄素甲醚的含量。方法:采用加热回流提取法制备供试品,采用高效液相色谱法测定首乌藤中二苯乙烯苷、大黄素、大黄素甲醚的含量。结果:首乌藤嫩、中、老枝中二苯乙烯苷含量分别为0.016、0.147和0.516%,大黄素含量分别为0.112、0.116、0.235 mg/g,大黄素甲醚的含量分别为0.299、0.584、0.747 mg/g。结论:首乌藤不同部位中二苯乙烯苷、大黄素及大黄素甲醚含量有明显差异,老枝中的含量大于中枝与嫩枝。三种成分在首乌藤不同部位含量变化趋势一致。采收时应注意保留老、中枝部分。

Study of effective components in different parts of Caulis Polygoni Multiflori

To study and compare contents of active ingredientsin different parts of Caulis PolygoniMultiflori, include 2,3, 5,4,-tetrahydroxy stilbene-2-O- β-D-glucoside( THSG) ,emodin and emodin-3-methyl ether. Heating reflux extrac-tion and preparation method was adopted for the test. Contents of THSG, emodin and emodin-3-methyl ether in Caulis PolygoniMultiflori wasdetermined by high performance liquid chromatography. Results: THSG content inshoots, normal branch and old branches of Caulis PolygoniMultiflori were 0. 016%, 0. 147% and 0. 516%, respectively. The content of emodin in shoots, normal branch and old branches of Caulis PolygoniMultiflori were 0.112, 0.116, 0.235 mg/g, respec-tively.The content of emodin-3-methyl ether in shoots, normal branch and old branches of Caulis PolygoniMultiflori were 0.299, 0.584, 0.747 mg/g, respectively. THSG, emodin and emodin-3-methyl ether contents in different parts of Caulis PolygoniMultiflori have significant difference. The content inold branches are more than that in shoots andnormal branch. The trend of three kinds of components in different parts of Caulis PolygoniMultifloriis consistent, thusthe old and normal branches should be harvested intentionally.