| 木豆不定芽再生途径的高效快繁体系 |
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| 引用本文： | 赵庆鹤,崔博文,文晓鹏.木豆不定芽再生途径的高效快繁体系[J].贵州大学学报(农业与生物科学版),2010,29(6):510-513. |
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| 作者姓名： | 赵庆鹤 崔博文 文晓鹏 |
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| 作者单位： | 贵州大学贵州省农业生物工程重点实验室; |
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| 摘 要： | 以木豆成熟胚为外植体,通过探讨不同培养条件对不定芽的诱导、增殖及生根等能力的影响,以建立高效的快繁体系。结果表明：适合胚生长的培养基为MS附加0.5 mg/L BA和0.1 mg/L IBA;不定芽增殖培养基以EC6附加0.4 mg/LBA与0.2 mg/L IBA效果最佳;生根培养基以MS附加0.1 mg/L IBA效果最好,生根率为82.4%,移栽成活率为85%。
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| 关 键 词： | 木豆 胚 不定芽 再生 离体快繁 |
An Efficient Protocol for Micropropagation of Pigeon Pea(Cajanus cajan)via Adventitious Bud Regeneration |
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| ZHAO Qing-he,CUI Bo-wen,WEN Xiao-peng.An Efficient Protocol for Micropropagation of Pigeon Pea(Cajanus cajan)via Adventitious Bud Regeneration[J].Journal of Mountain Agriculture & Biology,2010,29(6):510-513. |
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| Authors: | ZHAO Qing-he CUI Bo-wen WEN Xiao-peng |
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| Institution: | ZHAO Qing-he,CUI Bo-wen,WEN Xiao-peng(Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang Guizhou 550025,China) |
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| Abstract: | Mature embryos of pigeon pea(Cajanus cajan) were used as the explants to investigate the establishment of regeneration system in the current work.The results showed that the best medium for embryo culture was MS supplemented with 0.5 mg/L BA and 0.1 mg/L IBA.EC6 medium supplemented with 0.4 mg/L BA and 0.2 mg/L IBA gave the best multiplication efficiency of plant.The MS medium supplemented with 0.1 mg/L IBA was the best for inducing roots from shoots,the rooting rate was up to 82.4%.The survival rate was ab... |
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| Keywords: | Pigeon pea(Cajanus cajan) embryo Adventitious bud regeneration Micropropagation |
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