| 生长调节物质对半夏人工种胚形成的影响 |
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| 引用本文： | 申起飞,;张明生,;李立青,;刘贵贤.生长调节物质对半夏人工种胚形成的影响[J].贵州大学学报(农业与生物科学版),2014(3):33-36. |
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| 作者姓名： | 申起飞 ;张明生 ;李立青 ;刘贵贤 |
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| 作者单位： | [1]贵州大学生命科学学院,贵州贵阳550025; [2]贵州大学山地植物资源保护与种质创新省部共建教育部重点实验室,贵州贵阳550025 |
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| 基金项目： | 贵州省中药现代化科技产业研究开发专项项目[黔科合中药字（2011）5065号];贵州省中药材现代产业技术体系建设项目（GZ-CYTX-02）. |
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| 摘 要： | 以半夏叶片为外植体,研究了生长调节物质6-BA(6-苄氨基腺嘌呤)、2,4-D(2,4-二氯苯氧乙酸)、α-NAA(α-萘乙酸)、IBA(吲哚丁酸)对半夏人工种胚诱导和增殖的影响。结果表明,6-BA和2,4-D有利于半夏叶片愈伤组织的诱导和增殖,适宜培养基为MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L;6-BA、IBA和NAA对半夏人工种胚的诱导和增殖效果较好,适宜培养基为MS+6-BA 1.0 mg/L+IBA 2.0 mg/L或MS+6-BA1.0 mg/L+NAA 2.0 mg/L。结果为对半夏人工种胚诱导及工厂化提供了试验依据。
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| 关 键 词： | 半夏 愈伤组织 快繁 脱分化 |
The Effects of Plant Growth Regulators on Artificial Embryo Formation of Pinellia ternata |
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| Institution: | SHEN Qi-fei , ZHANG Ming-sheng, LI Li-qing , LIU Gui-xian ( 1. College of Life Sciences, Guizhou University, Guiyang Guizhou 550025, China; 2. Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guizhou University, Guiyang Guizhou 550025, China) |
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| Abstract: | The effects of 6 - benzyl amino adenine （6 - BA）, 2,4 - dichlorobenzene oxygen ethanoic acid （2,4 -D）, alpha naphthyl acetic acid （ct -NAA） and indole butyric acid （IBA） on the induction and pro- liferation of artificial embryos of Pinellia ternata were investigated herein using the leaves as the explants. The results showed that 6 - BA and 2,4 - D were advantageous for callus induction and multiplication, and the best medium was MS ＋ 6 - BA 1.0 mg/L ＋ 2,4 - D 2.0 mg/L. The 6 - BA, IBA and NAA were suitable for the induction and proliferation of artificial embryos, and the appropriate medium was MS ＋ 6 - BA 1.0 mg/L ＋ IBA 2.0 mg/L, or MS ＋ 6 - BA 1.0 mg/L ＋ NAA 2.0 mg/L. The results may facilitate the production of artificial seeds for this medical plant. |
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| Keywords: | Pinellia ternata callus rapid propagation dedifferentiation |
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