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磺胺类抗生素对青海弧菌Q67的浓度比依赖性拮抗作用
引用本文:丁婷婷,张瑾,董欣琪,洪桂云,鲍立宁.磺胺类抗生素对青海弧菌Q67的浓度比依赖性拮抗作用[J].农业环境科学学报,2017,36(11):2199-2206.
作者姓名:丁婷婷  张瑾  董欣琪  洪桂云  鲍立宁
作者单位:1. 安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥 230601;2. 安徽建筑大学环境与能源工程学院,安徽省水污染控制与废水资源化重点实验室,合肥 230601;清华大学新兴有机污染物控制北京市重点实验室,北京 100084
基金项目:国家自然科学基金项目(21677001,21207002);安徽省自然科学基金项目(1708085MB50)
摘    要:为系统考察磺胺类抗生素药物(SAs)污染物长期暴露下的生物毒性效应,以5种磺胺类抗生素(SAs):磺胺氯哒嗪(SCP)、磺胺嘧啶(SD)、磺胺吡啶(SPY)、磺胺甲恶唑(SMX)和磺胺二甲嘧啶(SM2)为研究对象,应用直接均分射线法(Equ-Ray)设计10个二元抗生素混合物体系(每个二元混合物体系设计5条具有不同浓度比的射线),应用优化的长期微板毒性分析法(L-MTA)系统测试这些抗生素在16 h对发光菌青海弧菌(Vibrio-qinghaiensis sp.-Q67,Q67)的发光抑制毒性,并应用浓度加和模型(CA)分析混合物毒性相互作用。结果表明:5种SAs及其混合物射线对Q67在16 h呈现明显的毒性,但不同的抗生素毒性大小不同,以半数效应浓度的负对数(pEC_(50))为毒性大小指标,5种SAs的毒性大小顺序为:SMX(p EC50=3.95)SCP(p EC50=3.65)SPY(pEC_(50)=3.41)SD(pEC_(50)=3.36)SM2(pEC_(50)=3.21);10个SAs的二元混合物体系中7个呈现出加和作用,3个呈现出拮抗作用;3个混合物体系拮抗作用随组分浓度比的变化呈现不同的变化规律:混合物体系SCP-SPY和SCP-SMX中的射线拮抗作用均随SCP浓度比逐渐减小,即从R1到R2逐渐明显,从R2到R5几乎不变,而SCP-SM2体系,拮抗作用随组分SCP的浓度比逐渐减小,即从R1到R2逐渐明显,从R2到R5,逐渐变得不明显,R5在较高浓度区甚至出现了协同作用。

关 键 词:磺胺类抗生素  混合物  青海弧菌  浓度比依赖性  拮抗作用
收稿时间:2017/5/17 0:00:00

Concentration-ratio-dependent antagonism of sulfonamide antibiotics towards Vibrio qinghaiensis sp.-Q67
DING Ting-ting,ZHANG Jin,DONG Xin-qi,HONG Gui-yun and BAO Li-ning.Concentration-ratio-dependent antagonism of sulfonamide antibiotics towards Vibrio qinghaiensis sp.-Q67[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2017,36(11):2199-2206.
Authors:DING Ting-ting  ZHANG Jin  DONG Xin-qi  HONG Gui-yun and BAO Li-ning
Institution:Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China,Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China;Beijing Key Laboratory for Emerging Organic Contaminants Control, Tsinghua University, Beijing 100084, China,Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China,Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China and Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China
Abstract:To investigate the long-term toxicity of sulfonamide antibiotics(SAs) towards organisms, five SAs:sulfamethazine(SCP), sulfamethoxazole(SD), sulfasalazine(SPY), sulfadiazine(SMX), and sulfachloro pyridazine(SM2) were selected as research subjects. The direct equipartition ray method was used to design ten binary mixture systems, and five rays with different concentration ratios(pis) were arranged in each mixture system. The toxicities of the five SAs and their mixture systems towards the photobacterium Vibrio qinghaiensis sp.-Q67 were determined using the optimized long-term microplate toxicity analysis(L-MTA) method. Concentration addition was employed as the standard reference model to analyze the toxicity interaction within mixture systems. The results showed that the toxicities of the five SAs and their mixture rays displayed obvious differences after 16 h. Based on the negative logarithm of median effect concentration(pEC50), the toxicity order of the five SAs was as follows:SMX(pEC50=3.95) > SCP(pEC50=3.65) > SPY(pEC50=3.41) > SD(pEC50=3.36) > SM2(pEC50=3.21). Seven out of the ten mixture systems displayed additive action and the other three, SCP-SPY, SCP-SMX, and SCP-SM2, showed different antagonism. Antagonism of SCP-SPY and SCP-SMX gradually strengthened with the decrease of component SCP''s pis from R1 to R2, and then was mostly stable from R2 to R5. The antagonism of the SCP-SM2 mixture system strengthened with the decrease of SCP''s pis from R1 to R2, then declined gradually from R2 to R5, and synergism appeared in the relatively high concentration region of R5.
Keywords:sulfonamide antibiotics  mixture  Vibrio qinghaiensis sp  -Q67  concentration-ratio dependency  antagonism
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