首页 | 本学科首页   官方微博 | 高级检索  
     检索      

砷胁迫下3-吲哚乙酸对不同砷富集能力植物根系形态和生理的影响
引用本文:和淑娟,王宏镔,王海娟,赵宾,李勤椿.砷胁迫下3-吲哚乙酸对不同砷富集能力植物根系形态和生理的影响[J].农业环境科学学报,2016,35(5):878-885.
作者姓名:和淑娟  王宏镔  王海娟  赵宾  李勤椿
作者单位:昆明理工大学环境科学与工程学院,昆明,650500
基金项目:国家自然科学基金项目(31360132);云南省中青年学术和技术带头人后备人才项目(2012HB007);昆明理工大学人才培养基金项目(KKZ3201222024);国家国际科技合作专项(0S2014ZR0062)
摘    要:采用室内水培法,研究了2 mg·L~(-1)五价砷胁迫下,外源添加不同浓度(0、10、20、40、60 mg·L~(-1))3-吲哚乙酸(IAA)14 d对砷超富集植物大叶井口边草(Pteris cretica var.nervosa)和同属的非超富集植物剑叶凤尾蕨(Pteris ensiformis)株高、生物量、砷含量、根系形态(根长、根尖数、根表面积)、根系活力、根细胞质膜ATPase活性、抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT、过氧化物酶POD)活性以及细胞膜脂过氧化产物丙二醛(MDA)含量的影响,并用逐步回归法分析了根部砷含量与所测定根系形态和生理指标的关系。结果表明:20 mg·L~(-1)IAA处理下,两种植物的生物量与对照相比显著增加,大叶井口边草叶片和叶柄砷含量、根系活力和根细胞质膜ATPase酶活性显著增加,且明显高于剑叶凤尾蕨;20~60 mg·L~(-1)IAA处理下,大叶井口边草根中的CAT、SOD和POD活性显著增加,而剑叶凤尾蕨根中的CAT活性显著降低,SOD活性显著增加,POD活性则无显著变化;20~40 mg·L~(-1)IAA处理下,两种植物根中的MDA含量显著降低,但大叶井口边草显著低于剑叶凤尾蕨。逐步回归分析结果显示,大叶井口边草根中砷含量与根系活力显著正相关,而剑叶凤尾蕨根中砷含量与各变量无关。因此,IAA的添加促使大叶井口边草保持较高的根系活力,进而有助于其超量富集砷。

关 键 词:3-吲哚乙酸  超富集植物    根系  根系活力
收稿时间:2015/12/16 0:00:00

Effects of indole-3-acetic acid on morphologic and physiological characteristics of root systems of plants with different arsenic-accumulating abilities under As stress
HE Shu-juan,WANG Hong-bin,WANG Hai-juan,ZHAO Bin and LI Qin-chun.Effects of indole-3-acetic acid on morphologic and physiological characteristics of root systems of plants with different arsenic-accumulating abilities under As stress[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2016,35(5):878-885.
Authors:HE Shu-juan  WANG Hong-bin  WANG Hai-juan  ZHAO Bin and LI Qin-chun
Institution:Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China,Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China,Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China,Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China and Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China
Abstract:A hydroponic experiment was conducted to investigate the effects of indole-3-acetic acid(IAA) on growth, root morphology, root activity, root ATPase activity in cytoplasmic membrane, root antioxidative enzymes(superoxide dismutase SOD, catalase CAT and proxidase POD), malondialdehyde(MDA, a peroxidation product of membrane lipids) content, and As accumulation in plants with different As-accumulating abilities. Arsenic hyperaccumulator(Pteris cretica var. nervosa) and non-hyperaccumulator(Pteris ensiformis) were exposed to 2 mg·L-1 As(V) and different concentrations of IAA(0,10,20,40,and 60 mg·L-1) for 14 d. Results showed that biomass of two plants significantly increased in the presence of 20 mg·L-1 IAA, compared with the control. At the 20 mg·L-1 IAA treatment, As concentrations in fronds and petioles, root activity, and root ATPase activity in cytoplasmic membrane of P. cretica var. nervosa increased significantly, and were significantly higher than those in P. ensiformis. At 20~60 mg·L-1 IAA treatments, the activities of three antioxidative enzymes(CAT, SOD and POD) in roots of P. cretica var. nervosa increased significantly, but the CAT activity decreased and SOD activities increased significantly in roots of P. ensiformis, in comparison with no IAA treatment. The POD activity showed no significant changes in the roots of P. ensiformis. The content of MDA in roots of both plants were significantly decreased by 20~40 mg·L-1 IAA treatments. However, MDA content in the roots of P. cretica var. nervosa was significantly lower than that of P. ensiformis. Correlation analysis showed that As concentrations in roots of P. cretica var. nervosa were positively related with root activity, whereas no correlation was found between root As concentrations and root parameters in P. ensiformis. Therefore, As hyperaccumulation of P. cretica var. nervosa was attributed to its high root activities.
Keywords:indole-3-acetic acid  hyperaccumulator  arsenic  root system  root activity
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《农业环境科学学报》浏览原始摘要信息
点击此处可从《农业环境科学学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号