铜绿微囊藻磷代谢过程研究

将铜绿微囊藻（Mierocystis aerugirtosa）接入不同磷浓度的培养基中进行光照培养，在分析水中溶解性磷浓度的同时，测定铜绿微囊藻的生长曲线和微囊藻中总磷、聚磷、可溶性磷以及糖原含量的变化过程，了解不同外源性磷浓度下铜绿微囊藻的生长和磷代谢过程。结果表明，在较高的初始磷浓度培养液中铜绿微囊藻的生长没有显著性差异．已不再限制微囊藻的生长。处于延迟期的铜绿微囊藻能从水环境中吸收外源性磷，在对数生长初期，藻利用体内的磷进行代谢，满足其生长的需要，即使外界还有较高的磷，铜绿微囊藻中总磷浓度也随着其生长而不断下降；在稳定期的初期微囊藻中可溶性磷含量达到最高值，藻中聚磷含量在对数期末明显增加，随后下降，而铜绿微囊藻中糖原含量在衰亡期显著增高，以细胞内聚磷变化趋势相反，从而与聚磷互补储存能量。

Phosphorus Metabolism of Microcystis aeruginosa During Its Growth Process

Microcystis aeruginosa was cultured in medium containing different phosphorus concentrations under light condition. The function parameters of cyanobacterium were measured including growth curve, total phosphorus contents, polyphosphate contents, and soluble phosphate contents in M. aeruginosa, as well as aqueous phosphorus concentrations, in order to explore phosphorus metabolism of M. aeruginosa under different phosphorus concentrations. The results showed that the growth of M. aeruginosa had little difference under the high initial phosphorus concentrations, and the aqueous phosphorus concentration was not the limited factor in our experiment because they were high enough for the growth of M. aeruginosa. And they may adsorb the exogenous phosphorus from the water circumstances at the lag period for growth of M. aeruginosa, it then use up intracellular phosphorus to grow in the early exponential phase, thus, total phosphorus decreased with the growth of M. aeruginosa. The intracellular soluble phosphorus was the highest at stationary phase, and there was an evident increase for the polyphosphate content in M. aerginosa at the late exponential phase. But, glucogen contents increased in the lag phase and the decline phase of M. aeruginosa. Chemical energy is deposited in glucogen and polyphosphate alternately for its survival.