草坪基质添加碳纳米材料对高羊茅生长和蚯蚓生理的影响

为探讨碳纳米材料对高羊茅生长和蚯蚓生理的影响,在草坪土壤基质中添加3种碳纳米材料(石墨烯、氧化石墨烯和碳纳米管),采用赤子爱胜蚓作为受试生物,研究了施加1%和3%碳纳米材料90 d后高羊茅生长、蚯蚓抗氧化酶活性和丙二醛(MDA)含量的变化。结果表明:不同比例的碳纳米材料对高羊茅的株高、地上鲜质量和干质量均没有显著影响。碳纳米材料均显著抑制了蚯蚓超氧化物歧化酶(SOD)的活性,3%碳纳米管的抑制率达到39.3%;暴露于3%氧化石墨烯,过氧化物酶(POD)活性显著降低;添加1%氧化石墨烯,过氧化氢酶(CAT)活性有所升高,添加3%碳纳米管和3%石墨烯,CAT活性显著低于1%氧化石墨烯处理。此外,碳纳米材料对蚯蚓体内的谷胱甘肽S-转移酶(GST)活性和MDA含量并无明显影响。因此,施加一定浓度的碳纳米材料不会影响草坪植物的生长,但其可以诱导蚯蚓体内活性氧(ROS)的产生,引起抗氧化酶活性发生变化,对土壤动物蚯蚓具有一定的毒性作用。

Effect of carbon nanomaterial addition to turf media on Festuca arundinacea growth and earthworm physiology

In this study, we investigated the effects of carbon nanomaterials on Festuca arundinacea growth, antioxidant enzyme activities, and malondialdehyde content in Eisenia foetida by adding three types of carbon nanomaterialsgraphene (G), graphene oxide (GO), and carbon nanotube (CNT)] to the turf media in different ratios (1% and 3%). Exposure to the carbon nanomaterials lasted for 90 days. We found no significant differences in plant height and shoot weights of Festuca arundinacea in the treatments with 1% and 3% of carbon nanomaterials. When earthworms were exposed to 1% and 3% of carbon nanomaterials, their superoxide dismutase (SOD) activity decreased significantly, with an inhibition rate of 39.3% in the treatment with 3% CNT. Peroxidase (POD) activity in Eisenia foetida decreased significantly, with an inhibition rate of 37.6% in the 3% GO treatment. Catalase (CAT) activity in Eisenia foetida increased in the 1% GO treatment, but decreased in treatments with 3% GNT and 3% G. In addition, we found no significant differences in Glutathione S-Transferase (GST) activity or malondialdehyde (MDA) content in earthworms exposed to carbon nanomaterials. These results suggest that a certain concentration of carbon nanomaterials will not affect turfgrass growth but can stimulate the production of ROS in earthworms and cause changes in the activities of antioxidant enzymes, indicating that carbon nanomaterials have some toxic effects on earthworms.