土著B[a]P降解菌群的富集及最佳降解条件研究

为了获得能够降解苯并a]芘(BaP)的微生物菌群,为生物修复多环芳烃污染的土壤提供菌种资源,本试验从长期石油污染的土壤中,富集了一个能够降解BaP的微生物菌群,并研究了其最佳降解条件,通过高通量测序研究了其群落结构。该菌群可以在15 d内将30 mg·L^-1的BaP降解33.34%。高通量测序的结果表明,该菌群主要由Bacillus、Zobellella、Gordonia和Rheinheimera组成,其中Bacillus是主要的降解菌。试验结果表明,该菌群的最佳降解条件为1%盐度,酵母浓度为80 mg·L^-1,pH值7.0。该菌群对芘、菲、荧蒽等多环芳烃也有一定的降解效果,其中荧蒽的降解效果最好,降解率达到99%。试验结果表明,该菌群在实际应用中具有很大的潜力。

Enrichment and degradation characteristics of an indigenous benzo[a]pyrene-degrading bacterial consortium

To obtain microorganisms for the bioremediation of PAH-contaminated soil, a bacterial consortium capable of degrading benzoa] pyrene(BaP)was enriched from oil-contaminated soil. The optimal degradation conditions and the community structure were studied. The bacterial consortium could degrade 33.34% BaP(30 mg·L^-1)after 15 days of treatment. High-throughput sequencing results showed that the bacterial consortium consisted of Bacillus, Zobellella, Gordonia, and Rheinheimera. Bacillus was the primary decomposing bacterial genera present. The results showed that the optimum degradation conditions were 1% salinity, supplementation with 80 mg·L^-1 yeast extract, and pH 7.0. The bacterial consortium could degrade pyrene, phenanthrene, and fluoranthene. Fluoranthene was the substrate with the highest degradability(99%). The results showed that the bacterial consortium have considerable potential for practical application for PAH biodegradation.