| 油菜根系分泌物的GC-MS检测方法研究 |
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| 引用本文： | 张红,高亚军,安蓉.油菜根系分泌物的GC-MS检测方法研究[J].农业环境科学学报,2014,31(3):290-295. |
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| 作者姓名： | 张红 高亚军 安蓉 |
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| 作者单位： | 西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 陕西 杨凌 712100,西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 陕西 杨凌 712100,西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 陕西 杨凌 712100 |
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| 基金项目： | 西北农林科技大学大型仪器设备新功能开发项目(dysb130201);黄土高原土壤侵蚀与旱地农业国家重点实验室(K318009902-1310) |
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| 摘 要： | 为筛选建立BP-5MS毛细管柱检测油菜根系分泌物的方法,本文采用水培试验,利用CH2Cl2提取油菜幼苗的根系分泌物,通过GC-MS检测分离鉴定。结果表明,根据待测物质组分性质,采用合适的溶剂延迟,分阶段细化升温程序,检测出来的特征峰较多且分布密集,特征峰分离相对比较好。从检测结果看,油菜苗期根系分泌物主要包括烃类、醇类、酯类和酸类化合物,且烃类和醇类化合物的相对含量较高,这些物质大多数具有化感作用,对植物在逆境环境中的生长和发育有一定的影响。
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| 关 键 词： | 油菜 根系分泌物 GC-MS 化感作用 |
| 收稿时间： | 2013/11/21 0:00:00 |
Testing Method of Rape Root Exudates by GC-MS Analysis |
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| ZHANG Hong,GAO Ya-jun and AN Rong.Testing Method of Rape Root Exudates by GC-MS Analysis[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2014,31(3):290-295. |
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| Authors: | ZHANG Hong GAO Ya-jun and AN Rong |
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| Institution: | College of Resources and Environment, Northwest A&F University, Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Yangling 712100, China,College of Resources and Environment, Northwest A&F University, Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Yangling 712100, China and College of Resources and Environment, Northwest A&F University, Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Yangling 712100, China |
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| Abstract: | In order to establish a method for the detection of rape root exudates, the hydroponic experiment was conducted using CH2 Cl2 extract to detect, isolate and identify root exudates of rape seedlings by GC-MS. The results showed that the detected peaks had more densely distributed and relatively good separation of peaks according to the composition of the testing substance by using a suitable solvent delay stages and refining temperature program. From the results, rape seedling root exudates included hydrocarbons, alcohols, esters and acids, while compounds of hydrocarbons and alcohols had relatively high levels. Most of these substances had allelopathic effects which had a certain impact on the environment in adversity plant growth and development. |
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| Keywords: | rape root exudation GC-MS allelopathic effect |
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