| 邻苯二甲酸酯降解菌的筛选、降解特性及土壤修复研究 |
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| 引用本文： | 杨婧,郭楚玲,刘沙沙,党志,卢桂宁.邻苯二甲酸酯降解菌的筛选、降解特性及土壤修复研究[J].农业环境科学学报,2018,37(5):933-940. |
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| 作者姓名： | 杨婧 郭楚玲 刘沙沙 党志 卢桂宁 |
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| 作者单位： | 华南理工大学环境与能源学院;工业聚集区污染控制与生态修复教育部重点实验室;广东省环境风险防控与应急处置工程技术研究中心 |
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| 基金项目： | 广东省科技项目（2014A020217002，2016B020242004） |
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| 摘 要： | 为寻找高效邻苯二甲酸二(2-乙基己基)酯(DEHP)降解菌,采用富集培养法从城市污水处理厂活性污泥中分离筛选出一株DEHP降解菌并命名为ASW6D。通过扫描电镜、16S r RNA同源性序列分析,初步将菌株ASW6D鉴定为分枝杆菌属(Mycobacterium sp.)。菌株ASW6D可在较宽温度(20~40℃)和pH(5~10)范围下高效降解DEHP,其最适生长降解条件为30℃、pH 8.0,3 d内可将初始浓度为500 mg·L~(-1)的DEHP降解82.87%。进一步采用GC-MS分析DEHP降解的中间产物,推测出DEHP的生物代谢途径为先通过β-氧化缩短DEHP侧链,生成邻苯二甲酸二丁酯(DBP),再将DBP转化为邻苯二甲酸(PA)。将菌株ASW6D接种到DEHP污染的土壤,可将土壤中DEHP去除率提高58.67%,表明ASW6D在PAEs污染环境生物修复方面的应用具有一定的潜力。
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| 关 键 词： | DEHP 分枝杆菌 代谢途径 污染土壤 |
| 收稿时间： | 2017/12/7 0:00:00 |
Isolation and characterization of phthalate-degrading bacterial strain, and its application in phthalate-contaminated soil |
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| YANG Jing,GUO Chu-ling,LIU Sha-sh,DANG Zhi and LU Gui-ning.Isolation and characterization of phthalate-degrading bacterial strain, and its application in phthalate-contaminated soil[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2018,37(5):933-940. |
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| Authors: | YANG Jing GUO Chu-ling LIU Sha-sh DANG Zhi and LU Gui-ning |
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| Institution: | School of Environment and Energy, South China University of Technology, Guangzhou 510006, China,School of Environment and Energy, South China University of Technology, Guangzhou 510006, China;The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China;Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou 510006, China,School of Environment and Energy, South China University of Technology, Guangzhou 510006, China,School of Environment and Energy, South China University of Technology, Guangzhou 510006, China;The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China and School of Environment and Energy, South China University of Technology, Guangzhou 510006, China;The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | DEHP Mycobacterium sp degradation pathway contaminated soil |
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