| 水稻根际促生菌的筛选鉴定及促生能力分析 |
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| 引用本文： | 刘泽平,王志刚,徐伟慧,陈文晶,吕智航,王春龙,史一.水稻根际促生菌的筛选鉴定及促生能力分析[J].农业环境科学学报,2018,35(2):119-125. |
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| 作者姓名： | 刘泽平 王志刚 徐伟慧 陈文晶 吕智航 王春龙 史一 |
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| 作者单位： | 齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006;农业部华南都市农业重点实验室, 广州 510640,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006,齐齐哈尔大学生命科学与农林学院, 黑龙江 齐齐哈尔 161006 |
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| 基金项目： | 齐齐哈尔市科学技术计划项目（SFGG-201576）；农业部华南都市农业重点实验室开放课题（003） |
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| 摘 要： | 植物根际促生菌（Plant growth-promoting rhizobacteria，PGPR）可分泌植物生长激素，促进土壤养分循环，是生物肥料重要的种质资源。本文从水稻根际土壤分离纯化根际促生菌，进行菌株鉴定，测定其促生能力。经过16S rDNA测序比对，筛选得到解磷菌4株（Bacillus pumilus LZP02，Bacillus aryabhattai LZP08，Staphylococcus epidermidis LZP10，Bacillus ginsengisoli LZP05），溶磷菌3株（Bacillus megaterium LZP03，Bacillus oryzaecorticis LZP04，Bacillus ginsengisoli LZP07），解钾菌3株（Bacillus aryabhattai LZP01，Bacillus subtilis LZP06，Bacillus licheniformis LZP09）。养分转化能力测试结果表明，Bacillus aryabhattai LZP01和Bacillus subtilis LZP06解钾能力较好；Bacillus pumilus LZP02和Bacillus huizhouensis LZP05解磷能力较强；Bacillus megaterium LZP03和Bacillusginsengisoli LZP07溶磷能力较好。对养分转化能力较强的菌株进行激素分泌能力测定，结果表明6种菌株均能产生生长素、赤霉素，均具有合成铁载体的能力。综合分析菌株养分转化与激素分泌能力发现，Bacillus megaterium LZP03、Bacillus huizhouensis LZP05和Bacillus subtilis LZP06促生能力较强，具有较强的开发利用潜力。研究成果为水稻微生物肥料的开发与生产提供了理论和技术支持。
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| 关 键 词： | 根际促生菌，水稻，赤霉素，吲哚乙酸，铁载体 |
| 收稿时间： | 2017/10/16 0:00:00 |
| 修稿时间： | 2017/12/11 0:00:00 |
Screen, Identification and Analysis on the Growth-Promoting Ability for the Rice Growth-Promoting Rhizobacteria |
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| LIU Ze-ping,WANG Zhi-gang,XU Wei-hui,CHEN Wen-jing,L&#; Zhi-hang,WANG Chun-long and SHI Yi-ran.Screen, Identification and Analysis on the Growth-Promoting Ability for the Rice Growth-Promoting Rhizobacteria[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2018,35(2):119-125. |
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| Authors: | LIU Ze-ping WANG Zhi-gang XU Wei-hui CHEN Wen-jing L&#; Zhi-hang WANG Chun-long and SHI Yi-ran |
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| Institution: | College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China;Key Laboratory of Urban Agriculture, Southern China, Ministry of Agriculture, Guangzhou 510640, China,College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China,College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China and College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China |
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| Abstract: | Plant growth-promoting rhizobacteria(PGPR)can secrete the growth hormone and promote soil nutrient cycling, thus, is an important germplasm resource of bio -fertilizer. In this study, the PGPR was isolated from the rice rhizosphere. According to 16S rDNA sequences, 10 strains were identifed, including 4 organic phosphorus bacteria (Bacillus pumilus LZP02, Bacillus aryabhattai LZP08, Staphylococcus epidermidis LZP10, Bacillus ginsengisoli LZP05), 3 inorganic phosphorus bacteria(Bacillus megaterium LZP03, Bacillus oryzaecorticis LZP04, Bacillus ginsengisoli LZP07)and 3 potassium bacteria(Bacillus aryabhattai LZP01, Bacillus subtilis LZP06, Bacillus licheniformis LZP09). The results from nutrient conversion analysis showed that Bacillus aryabhattai LZP01 and Bacillus subtilis LZP06 performed better on the potassium releasing ability. Bacillus pumilus LZP02 and Bacillus huizhouensis LZP05 performed better on the function of organic phosphorus. Bacillus megaterium LZP03 and Bacillus ginsengisoli LZP07 performed better on the function of inorganic phosphorus. Further, the hormone secretion capacity was measured for these 6 strains. The results showed that all 6 strains could produce auxin and gibberellin, and had the ability to synthesize iron carrier. Moreover, the results showed that Bacillus megaterium LZP03, Bacillus huizhouensis LZP05 and Bacillus subtilis LZP06 had stronger ability to promote the nutrient conversion and hormone secretion. Systematically, we believe that these three strains have great potential application on microbial fertilizer. |
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| Keywords: | root promoting bacteria rice gibberellin indole acetic acid iron carrier |
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