莲子心总黄酮的大孔吸附树脂纯化及其抑菌活性

为优选莲子心总黄酮的纯化方法,测试其抑菌活性,采用X-5型大孔吸附树脂对莲子心总黄酮进行纯化工艺研究,并利用杯碟法,以芦丁为对照,研究了莲子心总黄酮的抑菌活性.结果表明,X-5型大孔吸附树脂纯化莲子心总黄酮的最佳工艺为：上样液含量为5.1 mg·mL-1,上样液pH为6,上样量为2 BV,洗脱剂为4 BV 50％乙醇,洗脱剂流速为2 BV·h-1.纯化后莲子心总黄酮的纯度达50％.莲子心总黄酮对霉菌（青霉、根霉）无明显的抑菌作用,但对细菌（金黄色葡萄球菌、沙门氏菌、大肠杆菌、假单胞杆菌和枯草芽孢杆菌）的抑菌作用明显,并获得最低抑菌浓度.

Macroporous resin purification and bacteriostasis of total flavonoids from lotus-seed plumule

The main purpose of this paper is to optimize purification conditions and prove antibacterial activity of total flavonoids from lotus-seed plumule.The purification conditions and antibacterial activity of total fiavonoids from lotus plumule were studied using X-5 resin and cylinder plate method,which was compared with the effect of rutin.The optimization of purification conditions of X-5 resin was concluded as follows：sample concentration about 5.1 mg · mL-1,pH 6,the sample flow rate 2 BV · h-1,the sample quantity of 2 BV,eluent of 50% ethanol solution,eluent flow rate of 2 BV · h-1,eluent quantity of 4 BV.Then the pudty of total flavonoids was 50%.It was showed that the total flavonoids from lotus-seed plumule had no antibacterial activity against Penicillium and Rhizopus.But it had a significantly positive inhibitory activity against bacteria （including Staphylococcus aureus,Escherichia coli,Bacillus subtilis,Pseudomonas,Salmonella）.