壳聚糖复合生物保鲜剂对冷藏带鱼保鲜效果的优化配比

在单一生物保鲜剂试验研究的基础上,通过3因素3水平的L9（33）正交试验,将壳聚糖、溶菌酶和茶多酚进行复配,以感官评分、挥发性盐基氮（TVB-N）含量、细菌总数、硫代巴比妥酸（TBA）含量和pH等作为评价指标,评定带鱼冷藏期间的品质变化,从中筛选保鲜效果最佳的复合型生物保鲜剂.结果表明：各因素对冷藏带鱼保鲜效果影响的主次顺序为壳聚糖、溶菌酶、茶多酚;10.0 g.L-1壳聚糖、0.3 g.L-1溶菌酶和3.0 g.L-1茶多酚为复合保鲜剂的最佳配比;带鱼经最佳配比的复合保鲜剂处理后,在（4±1）℃下贮藏,其TVB-N含量、细菌总数、TBA含量和pH明显低于未经处理的冷藏对照组,感官值也显著优于对照组,冷藏货架期可延长11-13 d;经后期成本核算,处理1 kg带鱼仅需0.24元,具有良好的应用推广前景.

Optimized proportion of chitosan complex biological fresh-keeping agents for the preservation of Trichiurus haumela under the cold storage

On the base of the research of single biological fresh-keeping agent,the orthogonal experiment was made to investigate the fresh-keeping effects of complex biological fresh-keeping agents（a mixture of chitosan,lysozyme and tea polyphenol） in the preservation of Trichiurus haumela under the cold storage（4±1 ℃）.The sensory scores,total volatile basic nitrogen（TVB-N）,total bacterial count,2-thiobarbituric acid（TBA） and pH values were used to evaluate its quality.According to the results of orthogonal experiment L9（33）,the order influenced was： chitosanlysozymetea polyphenol,and the optimum composition of chitosan 10.0 g·L-1,lysozyme 0.3 g·L-1 and tea polyphenol 3.0 g·L-1 was determined.It showed that total bacterial count,TVB-N,TBA and pH value of T.haumela processed by complex biological fresh-keeping agents were much lower than those of the controlled,and had the higher sensory evaluation values.The shelf-life of T.haumela treated by the complex biological fresh-keeping agents could be extended 11-13 days.The cost of processing one kilogram T.haumela was only 0.24 yuan,which have great application prospects.