农药残留检测用植物酯酶筛选研究 |
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引用本文: | 刘洋,赵红艳,刘如芬,诸正典.农药残留检测用植物酯酶筛选研究[J].安徽农学通报,2012(19):177-179. |
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作者姓名: | 刘洋 赵红艳 刘如芬 诸正典 |
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作者单位: | 曲靖师范学院化学化工学院,云南曲靖655011 |
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基金项目: | 曲靖师范学院分析化学重点课程仪器分析重点课(TD200901);曲靖师范学院综合性设计性实验项目(Syjx2011014). |
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摘 要: | 目的:选用4种块茎马铃薯、红薯、茨菇、芋头提取植物酯酶,研究其检测有机磷农药的最佳条件,拟选取适宜的植物酶源;方法:使用酶抑制法,研究4种块茎中提取出的植物酯酶对农药敌敌畏、敌百虫、甲胺磷、辛硫磷灵敏度及其最低检测限;结果:4种植物酯酶均对农药敌百虫的灵敏度最好,最高可达37.184,检测限也低于国家标准;结论:因此,该酶可作为新的有机磷农药残留检测用酶。
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关 键 词: | 农药残留 植物酯酶 有机磷农药 抑制率 |
Selection of Phytoesterases and Reaction Conditions for Using Plant Esterases to Detect Pesticide Residues |
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Institution: | Liu Yang et al. (College of Chemistry and Chemical Engineering, Qujing Normal University,Qujing 655001 ,China) |
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Abstract: | Objective : Using Plant esterase obtain Four tuber (Solanum tuberosum L, lpomoea batatas, Sagittaria sagittifolia, Colocasia esculenta (L.) Schoot),This article reports optimal condition for detection of Organophosphorus Pesticide Resi- due. in order to select the optimal esterase for the detection ; Method:Based on the inhibiting mechanism of enzyme, phy- toesterases from various plant sources are investigated the sensitivity and the limit of detectable to dichlorvos (DDVP) .dip- terex,trichlorphon . Methamidophos, phoxim. Results:The results showed that the limits of detection were lower than the maximum of residue limit ,which meets the national standard of detection. Conclusion:The results indicated that the esterase obtained from Ipomoea and Colocasia esculenta make an optimal enzyme source for detecting agricultural pesticides residue. |
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Keywords: | Pesticide residue Plant esterase Organic phosphorus pesticide Inhibition rate |
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