| 中国樱桃离体叶片愈伤组织诱导的研究 |
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| 引用本文： | 彭丽萍,张远兵,胡正和.中国樱桃离体叶片愈伤组织诱导的研究[J].安徽农学通报,2010,16(17):51-53. |
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| 作者姓名： | 彭丽萍 张远兵 胡正和 |
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| 作者单位： | 安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100 |
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| 基金项目： | 安徽省教育厅优秀人才项目，安徽科技学院引进人才基金 |
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| 摘 要： | 以中国樱桃试管苗的离体叶片为外植体,MS为基本培养基,研究2,4-D和6-BA以及NAA和6-BA的不同浓度组合,以及叶片的不同部位、暗处理对愈伤组织诱导的影响。结果表明：培养基MS＋2.0mg/L 2,4-D＋2.0mg/L 6-BA＋3%蔗糖对愈伤组织的诱导最好,叶基部（包含部分叶柄）诱导率可达100%,暗处理5d,然后转入光照下培养可提高叶尖部和叶中部愈伤组织的诱导效果。
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| 关 键 词： | 中国樱桃 离体叶片 愈伤组织 诱导 |
Study on Inducing Callus of Vitro Leaves of Chinese Cherry |
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| Institution: | Peng Liping et al.(College of Life Sciences,Anhui Science and Technology University,Fengyang 233100,China) |
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| Abstract: | The vitro leaves of Chinese Cherry was as exphant,MS was basic medium.Studying on different concentrations of 2,4-D and 6-BA,NAA and 6-BA,different parts of the leaves and illumination time for effects of callus induction.The results showed that the medium MS＋2.0mg/L2,4-D＋2.0mg/L6-BA＋3% sucrose was best for callus induction,Callus induction rate of the base of leaves（including part of petiole） can amount to 100 %.The conducting of dark incubation for 5 days,and then moved to light cultivating can improve tip and leaves central effect of callus induction. |
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| Keywords: | Chinese Cherry Vitro leaves Callus Induction |
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