玫瑰天竺葵组培快繁技术体系的建立 |
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引用本文: | 鞠玉栋,杨敏,吴维坚,李珊珊.玫瑰天竺葵组培快繁技术体系的建立[J].安徽农学通报,2015(1):26-28. |
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作者姓名: | 鞠玉栋 杨敏 吴维坚 李珊珊 |
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作者单位: | 福建省农科院甘蔗研究所,福建漳州,363005 |
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摘 要: | 以玫瑰天竺葵茎段为外植体材料,研究其组培快繁技术体系。结果表明:初代诱导培养基以MS+BA1.0mg/L+NAA0.1mg/L为宜;继代培养基以MS+BA0.5mg/L+NAA0.05mg/L为宜,芽增殖倍数为5.8;生根培养基以1/2MS+IBAA0.6mg/L为宜,生根率达97.5%以上,平均生根数13.8条;移栽基质以泥炭土∶珍珠岩=3∶1为宜,成活率达98.3%。
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关 键 词: | 玫瑰天竺葵 茎段 组织培养 |
Establishment of Tissue Culture and Rapid Propagation System of Pelargonium roseu |
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Abstract: | The tissue culture and rapid propagation system of Pelargonium roseu is studied using stems as explants in this paper. The results showed that the suitable shoot-inducing medium was MS+BA1.0mg/L+NAA0.1mg/L,with inducement rate up to 96.7%.The suitable shoots multiplication culture medium was MS+BA0.5mg/L+NAA0.05mg/L,with multiplication coefficient of 5.8.The suitable rooting culture medium was 1/2MS+IBAA0.6mg/L,with rooting rate up to 97.5%,in which average number of roots was 13.8. The suitable trans?plant medium was peat soil∶perlite (V/V)=3∶1,with survival rate up to 98.3%. |
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Keywords: | Pelargonium roseu Stem Tissue culture |
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