| 一株产几丁质酶菌株的筛选及产酶条件研究 |
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| 引用本文： | 郑爱芳,魏和平,许远,芮辰飞,王枫,宋伟佳.一株产几丁质酶菌株的筛选及产酶条件研究[J].安徽农学通报,2010,16(20):42-43,62. |
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| 作者姓名： | 郑爱芳 魏和平 许远 芮辰飞 王枫 宋伟佳 |
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| 作者单位： | 安庆师范学院生命科学学院,安徽安庆,240611;安庆师范学院生命科学学院,安徽安庆,240611;安庆师范学院生命科学学院,安徽安庆,240611;安庆师范学院生命科学学院,安徽安庆,240611;安庆师范学院生命科学学院,安徽安庆,240611;安庆师范学院生命科学学院,安徽安庆,240611 |
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| 摘 要： | 用几丁质作为唯一碳源,从水稻田土中成功分离筛选出一株具有较强几丁质分泌能力的菌株JDZ1。通过单因素法对此菌株生长条件进行优化,结果表明最佳理化条件为:温度35℃、pH7.6,转速250rpm,发酵28h。同时,采用酶活力测定优化研究,结果显示,在温度为35℃、pH7.5条件下,酶活力最强。
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| 关 键 词： | 几丁质酶 筛选 酶活力 |
Study on Conditions for Producing Chitinase and Screening by a Chitinolytic Bacterium Strain |
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| Zheng Aifang,Wei Heping,Xu Yuan,Rui Chenfei,Wang Feng,Song Weijia.Study on Conditions for Producing Chitinase and Screening by a Chitinolytic Bacterium Strain[J].Auhui Agricultural Science Bulletin,2010,16(20):42-43,62. |
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| Authors: | Zheng Aifang Wei Heping Xu Yuan Rui Chenfei Wang Feng Song Weijia |
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| Institution: | Zheng Aifang Wei Heping Xu Yuan Rui Chenfei Wang Feng Song Weijia (School of Life Sciences, Anqing Normal University, AnQing246011,China) |
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| Abstract: | Chitin was used as sole carbon , screening a bacterium strain with great abilities of producing chitin from paddy field successfully. Optimization growth conditions of bacterium strain through single factor method. The optimum conditions shows that , 35℃ , pH 7.6, 250rpm for 28h.By the same time, research on optimization by enzyme activity determination, the condition of maximum enzyme activity with 35℃、pH 7.5. |
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| Keywords: | Chitinase Screening Enzyme activity |
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