液相色谱法测定饲料中氟喹诺酮类含量方法研究

以2％甲酸乙腈溶液为提取液，采用MCX固相萃取，荧光检测器检测，激发波长280nm，发射波长450nm，外标法定量，本项目建立了同时测定饲料中4种氟喹诺酮类药物的反相高效液相色谱分析方法。4种药物在5—500μg/ml浓度范围内呈良好的线性关系，相关系数R。大于0．99996。添加药物水平20--500μg／kg的回收率在75．6％一97．2％。环丙沙星、恩诺沙星、沙拉沙星的定量检出限为20μg／kg，达氟沙星为4μg／kg。试验结果表明：该方法快速简便，准确可靠，重现性好，可用于监测饲料中氟喹诺酮类含量。

Research on determination of fluoroquinolones in animal feed by liquid chromatography

A reversed-phase High Performance Liquid Chromatography method was developed for the simultaneous analysis of four fluoroquinolones in animal feed. The determination conditions were as follows： 2% formic acid in acetonitrile solution was used as extraction solvent,MCX solid phase ex- traction was used for extraction, fluorescence detector, excitation wavelength of 280ram, emission wave- length of 450mm,external standard method were used for determination. The linear range for the de- termination of four fluoroquinolones was 5--500μg/ml with R2〉0.9996, showing very good linear rela- tion. The recoveries laid in the range of 75.6%--97.2% at spiking level of 20--500μg/kg. The quanti- tative detection limit is 20μg/kg for ciprofloxacin,enrofloxacin and sarafloxacin and 4μg/kg for danofloxacin. The results show that this determination method is repid and simple,a ccurate and reli- able,good at reproducibility,which can be used to monitor the contents of fluoroquinolones in animal feed.